Methods in Membrane Lipids

Introduction to Membrane Lipids.- Introduction: Membrane properties (Good) for Life.- Introduction To Fluorescence Probing Of Biological Membranes.- The Assembly and Use of Tethered Bilayer Lipid Membranes (tBLMs).- Preparation of Detergent-Resistant Membranes (DRMs) from Cultured Mammalian Cells.- Isolation of Giant Plasma Membrane Vesicles for Evaluation of Plasma Membrane Structure and Protein Partitioning.- Asymmetric Giant Lipid Vesicle Fabrication.- Cholesterol Depletion using Methyl-b-cyclodextrin.- A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systems.- Imaging Membrane Order using Environmentally Sensitive Fluorophores.- 3D Super-resolution Imaging by Localisation Microscopy.- Electron Microscopy Methods for Studying Plasma Membranes.- Measuring Cytoskeleton and Cellular Membrane Mechanical Properties by Atomic Force Microscopy.- Fluorescence Linear Dichroism Imaging for Quantifying Membrane Order.- Scanning Fluorescence Correlation Spectroscopy on Biomembranes.- X-ray Diffraction of Lipid Model Membranes.- Solid State NMR of Lipid Model Membranes.- Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis and Applications.- The Laurdan Spectral Phasor Method To Explore Membrane Micro-Heterogeneity And Lipid Domains In Live Cells.- Cholesterol Behavior in Asymmetric Lipid Bilayers: Insights from Molecular Dynamics Simulations.- Computer Simulations Of Phase Separation In Lipid Bilayers and Monolayers.