Rhinoviruses
Humana Press Inc. (Verlag)
978-1-4939-1570-5 (ISBN)
Authoritative and easily accessible, Rhinoviruses: Methods and Protocols seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic approaches to combat HRV, and most importantly, its pathogenic effects in asthma exacerbation.
Classification and Evolution of Human Rhinoviruses.- Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples.- Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples.- Molecular Genotyping of Human Rhinovirus by PCR and Sanger Sequencing.- Growth of Human Rhinovirus in H1-Hela Cell Suspension Culture and Purification of Virions.- Propagation of Rhinovirus – C Strains in Differentiated Epithelial Cells of Human Airway at Air-Liquid Interface.- Infectivity Assays of Human Rhinovirus-A and –B Serotypes.- Application of FCS in Studies of Rhinovirus Receptor Binding and Uncoating.- Capillary Electrophoresis, Gas Phase Electrophoretic Mobility Molecular Analysis, and Electron Microscopy - Effective Tools for Quality Assessment and Basic Rhinovirus Research.- Proteases of Human Rhinovirus – Role in Infection.- A Protocol to Express and Isolate HRV16 3C Protease for Use in In vitro Protease Assays.- Reverse Genetics System for Studying Human Rhinovirus Infections.- Reverse Genetic Engineering of the Human Rhinovirus Serotype 16 Genome to Introduce an Antibody-Detectable Tag.- Mouse Models of Rhinovirus Infection and Airways Disease.
Reihe/Serie | Methods in Molecular Biology ; 1221 |
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Zusatzinfo | 17 Illustrations, color; 20 Illustrations, black and white; XII, 190 p. 37 illus., 17 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
Studium ► Querschnittsbereiche ► Infektiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | high-end imaging techniques • human rhinovirus • human rhinovirus research • molecular genotyping • protease assays • Reverse Genetics |
ISBN-10 | 1-4939-1570-3 / 1493915703 |
ISBN-13 | 978-1-4939-1570-5 / 9781493915705 |
Zustand | Neuware |
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