Adrenergic Receptor Protocols

Genetic Analysis.- Construction of Libraries for Isolation of Adrenergic Receptor Genes.- Isolation of Adrenergic Receptor Genes.- Analyses of Adrenergic Receptor Sequences.- Polymerase Chain Reaction Screening of Genomic Libraries for Adrenergic Receptor Genes.- Solution-Phase Library Screening for Identification of Rare Adrenergic Receptor Clones.- Genetic Polymorphisms of Adrenergic Receptors.- RNA Analysis.- Northern Blot Analyses Detecting Adrenergic Receptor mRNAs.- Ribonuclease Protection Assay for the Detection of ?1-Adrenergic Receptor RNA.- Determination of Adrenergic Receptor mRNAs by Quantitative Reverse Transcriptase-Polymerase Chain Reactions.- Nuclear Run-On Assays for Measurement of Adrenergic Receptor Transcription Rate.- Primer Extension Methods for Determination of ?1-Adrenergic Receptor mRNA Start Sites.- Expression Analysis.- Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors.- Expression of ?-Adrenergic Receptors in Recombinant Baculovirus-Infected Insect Cells.- Expression of ?-Adrenergic Receptors in E. coli.- Use and Pharmacological Analysis of Established and Transfected Cell Lines Expressing Adrenergic Receptors.- Transient Transfection and Adrenergic Receptor Promoter Analysis.- Antisense RNA/DNA-Based Techniques to Probe Adrenergic Receptor Function.- Targeted Disruption of Adrenergic Receptor Genes.- Receptor and Second Messenger Analysis.- Development of Antibodies to Adrenergic Receptors.- Western Blot Detection of Adrenergic Receptors.- UV Crosslinking of Adrenergic Receptors and Ligands.- Detection of ?-Adrenergic Receptors by Radioligand Binding.- Assessing Adrenergic Receptor Conformation Using Chemically Reactive Fluorescent Probes.- Biochemical Methods for Detection and Measurement of Cyclic AMP and AdenylylCyclase Activity.- Assay of Arachidonic Acid Release Coupled to ?1- and ?2-Adrenergic Receptors.- Patch-Clamp Recording Methods for Examining Adrenergic Regulation of Potassium Currents in Ocular Epithelial Cells.- Transactivator Analysis.- Southwestern Blots for Detection of a DNA Binding Protein Recognizing the ?1B-Adrenergic Receptor Gene Promoter.- DNase I Footprinting Analysis of Transcription Factors Recognizing Adrenergic Receptor Gene Promoter Sequences.- Electrophoretic Mobility Shift Assay for Detection of DNA Binding Proteins Recognizing ?-Adrenergic Receptor Gene Sequences.- Determination of mRNA Stability and Characterization of Proteins Interacting with Adrenergic Receptor mRNAs.- Microscopy Analysis.- Use of Immunohistochemistry and Confocal Microscopy in the Detection of Adrenergic Receptors.- Distribution of ?2A- and ?2C-Adrenergic Receptor Immunoreactivity in the Central Nervous System.- Quantitative Light Microscopic Autoradiography of ?2-Adrenergic Radioligand Binding Sites.- In Situ Hybridization of Adrenergic Receptor mRNA in Brain.- Use of Electron Microscopy in the Detection of Adrenergic Receptors.