Protein Aggregation in Bacteria (eBook)

SILVIA MARIA DOGLIA, PhD, is Professor of Physics at the University of Milano-Bicocca, Italy. She received her Laurea degree in Physics at the University of Milano. She has been Staff Research Scientist of the Italian National Research Council; Visiting Scientist at the University of Stockholm; Visiting Professor at the Universities of Orléans (Fr) and of Reims (Fr). Her research in Biophysics at the University of Milano-Bicocca is focused on the study of protein folding and aggregation in vitro and in situ. MARINA LOTTI, PhD, is Professor of Biochemistry at the University of Milano-Bicocca, Italy, where she leads the group Protein Engineering and Industrial Enzymology, and is the Head of the Department of Biotechnology and Biosciences. She obtained her PhD degree at the Max-Planck Institute of Molecular Genetics, Berlin, and was a researcher of the Italian National Research Council. Major research topics include the production of recombinant proteins, protein aggregation, cold-active enzymes, and intrinsically disordered proteins.

Contributors vii

Preface xi

Introduction to the Wiley Series in Protein and Peptide Science
xiii

1 Fundamentals of Protein Folding 1

Vladimir N. Uversky

2 Recruiting Unfolding Chaperones to Solubilize Misfolded
Recombinant Proteins 63

Rayees U.H. Mattoo and Pierre Goloubinoff

3 Osmolytes as Chemical Chaperones to Use in Protein
Biotechnology 77

Ario de Marco

4 Inclusion Bodies in the Study of Amyloid Aggregation 93

Anna Villar-Piqué and Salvador Ventura

5 Protein Aggregation in Unicellular Eukaryotes 117

Marina Caldara, Joris Winderickx, and Vanessa Franssens

6 Structural Properties of Bacterial Inclusion Bodies 151

Antonino Natalello, Diletta Ami, and Silvia Maria Doglia

7 Residue-Specific Structural Studies of Inclusion Bodies
181

Christian Wasmer, Marielle Wälti, Yongli Chen, and Lei
Wang

8 Biomedical Applications of Bacterial Inclusion Bodies
203

Imma Ratera, Spela Peternel, Joaquin Seras-Franzoso, Olivia
Cano-Garrido, Elena García-Fruitós, Rafael Cubarsí,
Esther Vazquez, José Luis Corchero, Escarlata
Rodríguez-Carmona, Jaume Veciana, and Antonio Villaverde

9 Aggregation of Recombinant Proteins: Understanding Basic
Issues to Overcome Production Bottlenecks 221

Marina Lotti and Loredano Pollegioni

10 Fusion to a Pull-Down Module: Designing Enzymes to Form
Biocatalytically Active Insoluble Aggregates 247

Bernd Nidetzky

Index 263