Extracellular Glycolipids of Yeasts

Degrees: Mr.Sci: July 2005 in Biology (Pushchino State University)Ph.D.: November 2006 in Biochemistry (Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences): Antifungal yeast glycolipids. (Supervisors Prof. Kulaev, I.S., and Dr. W.I.Golubev Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences)Positions held:2005-2008: Junior Researcher, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences2008-Present: Researcher Skryabin Institute of Biochemistry and Physiology of Microorganisms Degrees: Mr.Sci: July 1979 in Biochemistry (Moscow State University, 1979)Ph.D.: Marh 1985 in Biochemistry (Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences): ATPase and ion transport in yeast vacuoles. (Supervisor Prof. Kulaev, I.S., Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences)Dr. Sci. in Biochemistry (December, 2001) (Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences): Exopolyphosphatases of Saccharomyces cerevisiaePositions held:1984-1989 Junior Researcher, Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences1989-1992 Researcher Skryabin Institute of Biochemistry and Physiology of Microorganisms1993-2004 Senior Researcher, Skryabin Institute of Biochemistry and Physiology of Microorganisms2004-2005 Leading Researcher, Skryabin Institute of Biochemistry and Physiology of Microorganisms2005-Present: Head of Laboratory of Regulation of Biochemical Processes Skryabin Institute of Biochemistry and Physiology of Microorganisms

Introduction
Chapter 1: Occurrence and structure of fungal extracellular glycolipids
1.1. Glycolipid secretion in eumycetes
1.2. The structures of extracellular glycolipids of eumycetes
1.2.1. Cellobiose lipids
1.2.2. Mannosylerythritol lipids, MELs
1.2.3. Sophorolipids
1.2.4. Rare extracellular fungal glycolipids
Chapter 2: Methods of study of extracellular glycolipids
2.1. The culture media and methods to increase the yield of extracellular glycolipids
2.2. Purification methods
2.3. Structure study by NRM-spectroscopy and mass-spectrometry
2.4. Methods for studying physical and chemical properties, antifungal
and membrane-damaging activities
Chapter 3: Physical and chemical properties of extracellular glycolipids
3.1. Solubility
3.2. Stability
3.3. Molecular masses
3.4. Surface properties
3.5. Interaction with artificial membranes
Chapter 4: Biological activities of extracellular yeast glycolipids
4.1. Antifungal activity of cellobiose lipids
4.1.1. Discovery of antifungal activity of cellobiose lipids
4.1.2. Antifungal activities and structure peculiarities
4.2. Membrane – damaging activity of cellobiose lipids
4.3. Biological activities of MELs and sophorolipids
Chapter 5: Biosynthesis and degradation of extracellular yeast glycolipids
5.1. Biosynthesis of cellobiose lipids
5.2. Biosynthesis of sophorolipids
5.3. Biosynthesis of MELs
5.4. Degradation of extracellular yeast glycolipids
Chapter 6: Applied prospects of cellobiose lipids, mannosylerythritol lipids, and sophorolipids
6.1. Membrane-damaging agents for research purposes
6.2. Prospects for medicine, environmental protection, agriculture and industry
6.3. Commercial products containing yeast extracellular glycolipids
7. Supplement: selected techniques of purification and assay of extracellular yeast glycolipids
7.1. Culture media
7.2. Chromatography
7.5. Fungicide activity assay
7.6. Membrane-damaging activity assay
8. References