Micropatterning in Cell Biology, Part B

Matthieu Piel and his team develop microfabricated and microfluidic tools to quantitatively control the physical parameters of the cell’s environment and study how cells grow, divide and migrate. The team focused on how physical confinement, geometry and forces affect cell division and cell migration. The general aim of these studies is to draw a line between the physics of the active matter cells are made of and the behavior of cells in the complex environment of tissues, in the context of the immune response and tumor development.

Section 1 Micropatterning isolated cell components1 Mitotic Spindle Assembly on Chromatin Patterns Made with Deep UV Photochemistry2 Geometrical control of actin assembly and contractility3 Micropatterning Microtubules4 Micropatterned, Multicomponent Supported Lipid Bilayers for Cellular Systems5 Reconstituting Functional Microtubule-Barrier Interactions

Section 2 Dynamic micro-patterning6 Polyacrylamide Hydrogel Micropatterning7 Dynamic Photo-chemical Silane Micro-patterning8 Dynamic Photo-chemical Lipid Micropatterning for Manipulation of Non-adherent Mammalian Cells9 Thermosensitive Micropatterned Substrates10 Fabrication of Micropatterned Arrays of Gold Nanoparticles for Photothermal Manipulation of Living Cells11 A Reagent-based Dynamic Trigger for Cell Adhesion, Shape Change or Cocultures12 Cell Patterning by Micro-pattern Projection of UV Light Through Photo-induced Enhancement of Cell Adhesion (PIECA)

Section 3 Micropatterning for specific applications, from forces to yeasts13 Preparing Substrates Encoding Cell Patterning and Localized Intracellular Magnetic Particle Stimulus for High-throughput Experimentation14 Microfabricated Chambers as Force-Sensors for Probing Forces of Fungal Growth15 Visualizing Single Rod-shaped Fission Yeast Vertically in Micro-sized Holes on Agarose Pad Made by Soft-lithography16 Microfabricated Environments to Study Collective Cell Behaviors