Metalloproteins

Methods and Protocols
Buch | Hardcover
299 Seiten
2014
Humana Press Inc. (Verlag)
978-1-62703-793-8 (ISBN)

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Metalloproteins -
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Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, Mössbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.

Advances in Research on Metalloproteins.- Purification of O2-Sensitive Metalloproteins.- Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources.- Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins.- Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers.- Electrochemistry of Metalloproteins – Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1.- FTIR Spectroscopy of Metalloproteins.- Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy.- A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds.- Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR).- Mössbauer Spectroscopy.- X-Ray Absorption Spectroscopy of Metalloproteins.- X-Ray Crystallographic Studies of Metalloproteins.- Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds.- Density Functional Theory – Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups.

Reihe/Serie Methods in Molecular Biology ; 1122
Zusatzinfo 57 Illustrations, color; 32 Illustrations, black and white; X, 299 p. 89 illus., 57 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
Schlagworte bioinorganic compounds • iron-sulfur proteins • metalloenzyme research • Metalloproteins • spectroscopy
ISBN-10 1-62703-793-4 / 1627037934
ISBN-13 978-1-62703-793-8 / 9781627037938
Zustand Neuware
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