FRET - Förster Resonance Energy Transfer (eBook)

Dr. Igor L. Medintz obtained his B.S. and M.S in Forensic Science, followed by a Ph.D. degree in Molecular Biology in 1998 at the City University of New York. He carried out research as a postdoctoral fellow at the University of California Berkeley as well as at U.S. Naval Research Laboratory (NRL) in Washington, D.C. Since 2004, he has been a Research Biologist at NRL where he focuses on developing chemistries to interface nanomaterials with biology and understanding how nanoparticles engage in different types of energy transfer. Professor Niko Hildebrandt obtained a Diploma in Medical Physics in 2001 at the University of Applied Sciences Berlin and a Ph.D. degree in Physical Chemistry in 2007 at the University of Potsdam, where he also carried out postdoctoral research until 2008. From 2008 to 2010 he was head of the group NanoPolyPhotonics at the Fraunhofer Institute for Applied Polymer Research in Potsdam. Since 2010 he has been Full Professor at Université Paris-Sud, where he is leading the group of NanoBioPhotonics (www.nbp.ief.u-psud.fr) at the Institut d'Electronique Fondamentale with a research focus on time-resolved FRET spectroscopy and imaging for multiplexed nanobiosensing.

Preface

PART ONE: BACKGROUND, THEORY, AND CONCEPTS
How I Remember Theodor Förster
Remembering Robert Clegg and Elizabeth Jares-Erijman and Their Contributions to FRET
Förster Theory
Optimizing the Orientation Factor Kappa-Squared for More Accurate FRET Measurements
How to Apply FRET: From Experimental Design to Data Analysis
Materials for FRET Analysis: Beyond Traditional Dye-Dye Combinations

PART TWO: COMMON FRET TECHNIQUES/APPLICATIONS
In Vitro FRET Sensing, Diagnostics, and Personalized Medicine
Single-Molecule Applications
Implementation of FRET Technologies for Studying the Folding and Conformational Changes in Biological Structures
FRET-Based Cellular Sensing with Genetically Encoded Fluorescent Indicators

PART THREE: FRET WITH RECENTLY DEVELOPED MATERIALS
FRET with Fluorescent Proteins
Semiconductor Quantum Dots and FRET
Multistep FRET and Nanotechnology

PART FOUR: SUPPORTING INFORMATION AND CONCLUSIONS
Data
Outlook on FRET: The Future of Resonance Energy Transfer

Index