Molecular and Cellular Aspects of Muscle Contraction

I. Molecular Factors Influencing Cardiac and Smooth.- Myosin Light Chain Composition in Non-Failing Donor and End-Stage Failing Human Ventricular Myocardium.- Study of the Vertebrate MHC Multigene Family during Heart Development.- Myosin-binding Protein C (MYBP-C) in Cardiac Muscle and Contractility.- The Endocardial Endothelial Na+/K+ ATPase and Cardiac Contraction.- KLF5/BTEB2, a Krüppel-like Zinc-finger Type Transcription Factor, Mediates both Smooth Muscle Cell Activation and Cardiac Hypertrophy.- Regulation of the Rho Signaling Pathway by Excitatory Agonists in Vascular Smooth Muscle.- Human Heart Failure: Dilated Versus Familial Hypertrophic Cardiomyopathy.- Regulation of Smooth Muscle Contraction by Calcium, Monomeric GTPases of the Rho Subfamily, and their Effector Kinases.- II. Molecular Mechanism of Actin-Myosin Interaction.- Bio-nanomuscle Project: Contractile Properties of Single Actin Filaments in an A-band Motility Assay System.- Development of an Apparatus to Control Load by Electromagnet for a Motility System In Vitro.- High-Resolution Imaging of Myosin Motor in Action by a High-Speed Atomic Force Microscope.- Translation Step Size Measured in Single Sarcomeres and Single Filament Pairs.- Motor Function of Unconventional Myosin.- Switch 1 Opens on Strong Binding to Actin.- Gene Transfer of Troponin I Isoforms, Mutants and Chimeras.- A Hypothesis about Myosin Catalysis.- In Vitro Actomyosin Motility in Deuterium Oxide.- III. Molecular Basis for Regulatory Mechanism of Muscle.- NMR Structural Study of Troponin C C-terminal Domain Complexed with Troponin I Fragment from Akazara Scallop.- Calcium Binding to Troponin C as a Primary Step of the Regulation of Contraction.- Isolation and Characterization of 190K Protein from Aorta Smooth Muscle.- SeveralAspects of Calcium Regulator, Mechanisms Linked to Troponin.- IV. Structural Changes During Contraction.- X-ray Interference Evidence Concerning the Range of Cross-Bridge Movement, and Backbone Contributions to the Meridional Pattern.- Modeling Analysis of Myosin-Based Meridional X-ray Reflections from Frog Skeletal Muscles in Relaxed and Contracting States.- Myosin Filament Structure and Myosin Cross-bridge Dynamics in Fish and Insect Muscles.- Use of Sinusoidal Length Oscillations to Detect Myosin Conformation by Time-Resolved X-ray Diffraction.- Orientation and Motion of Myosin Light Chain and Troponin in Reconstituted Muscle Fibers as Detected by ESR with a New Bifunctional Spin-Label....- Molecular Organizations of Myofibrils of Skeletal Muscle Studied by Atomic Force Microscopy.- Three-dimensional Structural Analysis of Individual Myosin Heads under Various Functional States.- Helical Order in Myosin Filaments and Evidence for One Ligand Inducing Multiple Myosin Conformations.- Evidence for the Involvement of Myosin Subfragment 2 in Muscle Contraction.- V. Nonmuscle Motile Systems.- A Study of Lamellipodial Membrane Dynamics by Optical Trapping Technique: Implication of Motor Activity in Movements.- Actin Dynamics in Neuronal Growth Cone Revealed with a Polarized Light Microscopy.- Thermodynamic Mechano-Chemistry of Single Motor Enzymes.- VI. Excitation-Contraction Coupling.- Cooperative Activation of Skeletal and Cardiac Muscle.- Measurement of Force Developed by a Single Cardiac Myocyte Using Novel Carbon Fibers.- The Special Structure and Function of Troponin I in Regulation of Cardiac Contraction and Relaxation.- Pharmacological Calcium Sensitivity Modulation of Cardiac Myofilaments.- 20-Hydroxyeicosatetraenoic Acid Potentiates Contractile Activation of Canine Basilar Artery in Response to Stretch via Protein Kinase C?-Mediated Inhibition of Calcium-Activated Potassium Channel.- Distinct Contractile Systems for Electromechanical and Pharmacomechanical Coupling in Smooth Muscle.- VII. Muscle Mechanics.- Force Response to Stretches in Activated Frog Muscle Fibres at Low Tension.- Molecular Step(s) of Force Generation: Temperature-Perturbation Experiments on Muscle Fibres.- Rates of Force Generation in Drosophila Fast and Slow Muscle Types Have Opposite Responses to Phosphate.- Does Cross-Bridge Activation Determine the Time Course of Myofibrillar Relaxation?.- Inter-Sarcomere Dynamics in Muscle Fibres. A Negrected Subject ?.- Stretch-induced Force Enhancement and Stability of Skeletal Muscle Myofibrils.- Adaptations in Titin’s Spring Elements in Normal and Cardiomyopathic Hearts.- An X-ray Diffraction Study on Contraction of Rat Papillary Muscle with Different Afterloads.- VIII. Muscle Fatigue and Energetics.- Role of the T-system and the Na-K Pump on Fatigue Development in Phasic Skeletal Muscle.- Mitochondrial Ca2+ in Mouse Soleus Single Muscle Fibres in Response to Repeated Tetanic Contractions.- Cellular Mechanisms of Skeletal Muscle Fatigue.- Myofibrillar Determinants of Rate of Relaxation in Skinned Skeletal Muscle Fibers.- Force, Sarcomere Shortening Velocity and ATPase Activity.- Evidence for the High Mechanical Efficiency of Cross-Bridge Powerstroke in Skeletal Muscle.- Rate of Actomyosin ATP Hydrolysis Diminishes during Isometric Contraction.- Energy Storage during Stretch of Active Single Fibres.- Three Dimensional Ultrasound Analysis of Fascicle Orientation in Human Tibialis Anterior Muscle Enables Analysis of Macroscopic Torque at the Cellular Level.- General Discussion Part I.- Special Talk: The Beginning ofLow-Angle X-ray Diffraction Work on Living Muscles.- General Discussion Part II.- General Discussion Part III Chaired by R. C. Woledge.- Concluding Remarks: Progress in Determining the Detailed Mechanism of Muscle Contraction.- Participants Photos.- Participants.