Nuclear Pore Complexes and Nucleocytoplasmic Transport - Methods

1. Fifty Years of Nuclear Pores and Nucleocytoplasmic Transport Studies: Multiple Tools Revealing Complex Rules 2. Imaging Metazoan Nuclear Pore Complexes by Field Emission Scanning Electron Microscopy 3. Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno SEM 4. Exploring Nuclear Pore Complex Molecular Architecture by Immuno-Electron Microscopy using Xenopus Oocytes 5. Utilizing the Dyn2 Dimerization-Zipper as a Tool to Probe NPC Structure and Function 6. The use of Targeted Proteomics to Determine the Stoichiometry of Large Macromolecular Assemblies 7. A Pulse-Chase Epitope Labeling to Study Cellular Dynamics of Newly Synthesized Proteins: A Novel Strategy to Characterize NPC Biogenesis and Ribosome Maturation/Export 8. Analysis of Nuclear Reconstitution, Nuclear Envelope Assembly, and Nuclear Pore Assembly using Xenopus in vitro Assays 9. Xenopus in vitro Assays to Analyze the Function of Transmembrane Nucleoporins and Targeting of Inner Nuclear Membrane Proteins 10. Imaging the Assembly, Structure and Function of the Nuclear Pore Inside Cells 11. Cell-Fusion Method to Visualize Interphase Nuclear Pore (NPC) Formation 12. An in vitro System to Study Nuclear Envelope Breakdown 13. Modern Tools to Study Nuclear Pore Complexes and Nucleocytoplasmic Transport in Caenorhabditis elegans 14. Assessing Regulated Nuclear Transport in Saccharomyces cerevisiae 15. Analysis of Nucleocytoplasmic Transport in Digitonin-Permeabilized Cells under Different Cellular Conditions 16. Novel Approaches for the Identification of Nuclear Transport Receptor Substrates 17. NPC Mimics: Probing the Mechanism of Nucleocytoplasmic Transport 18. Analysis of RNA Transport in Xenopus Oocytes and Mammalian Cells 19. Strategies for Investigating Nuclear-Cytoplasmic tRNA Dynamics in Yeast and Mammalian Cells 20. Dissecting Ribosome Assembly and Transport in Budding Yeast 21. Approaches to Studying Subnuclear Organization and Gene-Nuclear Pore Interactions