Plant Proteomics
Humana Press Inc. (Verlag)
978-1-62703-630-6 (ISBN)
Authoritative and easily accessible, Plant Proteomics: Methods and Protocols, Second Edition seeks to serve both professionals and novices looking to exploit the full potential of proteomics in plant biology research.
Plant Proteomics Methods and Protocols.- From Proteomics to Systems Biology: MAPA, MASS WESTERN, PROMEX and COVAIN as a User-Oriented Platform.- Plant Proteomics: From Genome Sequencing to Proteome Databases and Repositories.- How to Use 2D Gel Electrophoresis in Plant Proteomics.- Standarization of Data Processing and Statistical Analysis in a 2-DE-Based Comparative Plant Proteomics Experiment.- The Expanding Universe of Mass Analyzer Configurations for Biological Analysis.- A Protocol for Protein Extraction from Lipid-Rich Plant Tissues Suitable for Electrophoresis.- Making a Protein Extract in Plant Pathogenic Fungi for Gel- and LC-Based Proteomics.- Plant Proteomics Methods to Reach Low-Abundance Proteins.- Combination of 2DE and LC for Plant Proteomics Analysis.- 2DE Analysis of Forest Tree Proteins Using Fluorescent Labels and Multiplexing.- Differential Plant Proteome Analysis by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ).- Global Quantitative Proteomics Using Spectral Counting: An Inexpensive Experimental and Bioinformatics Workflow for Deep Proteome Coverage.- Combining Chymotrypsin/trypsin Digestion to Identify Hydrophobic Proteins from Oil Bodies.- Mass Western for Absolute Quantification of Target Proteins and Considerations About the Instrument of Choice.- Selected Reaction Monitoring Mass Spectrometry: A Methodology Overview.- Mass Spectrometry Based Imaging of Metabolites and Proteins.- Using the Yeast Two-Hybrid System to Identify Protein-Protein Interactions.- Modifications of Wheat Germ Cell-Free System for Functional Proteomics of Plant Membrane Proteins.- Arabidopsis Proteomics: A Simple and Standardizable Workflow for Quantitative Proteome Characterization.- Analysis of Rice Proteins Using SDS-PAGE Shotgun Proteomics.- Medicago truncatula Proteomics for Systems Biology: Novel Rapid Shotgun LC-MS Approach for Relative Quantification Based on Full-Scan Selective Peptide Extraxtion (Selpex).- Soybean Proteomics.- Proteome Analysis of Orphan Plant Species, Fact or Fiction?.- An Improved Detergent-Compatible Gel-Fractionation LC-LTQ-Orbitrap-MS Workflow for Plant and Microbial Proteomics.- Seed Proteomics.- Back to Osborne. Sequential Protein Extraction and LC-MS for Characterization of the Seed Proteome.- Xylem Sap Proteomics.- Suspension Cultured-Plant Cells as a Tool to Analyze the Extracellular Proteome.- Pollen Cultivation and Preparation for Proteome Studies.- Isolation of the Plant Cytosolic Fraction for Proteomic Analysis.- Quantitative Proteomic Analysis of Intact Plastids.- Shotgun Proteomics of Plant Plasma Membrane and Microdomain Proteins Using Nano-LC-MS/MS.- Plant Mitochondrial Proteomics.- Separation of the Plant Golgi Apparatus and Endoplasmic Reticulum by Free-Flow Electrophoresis.- Isolation of Leaf Peroxisomes from Arabidopsis for Organelle Proteomics Analyses.- Proteomics of Field Samples, the Case of the Chernobyl Area.- Differential Proteome and Secretome Analysis During Rice-Pathogen Interaction.- Protein Extraction and Gel-Based Separation Methods to Analyse Responses to Pathogens in Carnation (Dianthus caryophyllus L).- StageTip-Based HAMMOC, an Efficient and Inexpensive Phosphopeptide Enrichment Method for Plant Shotgun Phosphoproteomics.- Simultaneous Identification and Quantification of Nitrosylation Sites by Combination of Biotin Switch and ICAT Labeling.- Tandem Metal-Oxide Affinity Chromatography for Enhanced Depth of Phosphoproteome Analysis.- N-Glycoprotein Enrichment by Lectin Affinity Chromatography.- Rapid and High-Throughput N-glycomic Analysis of Plant Glycoproteins.- Assay for Proteasome-Dependent Protein Degradation and Ubiquitinated Proteins.- Separation of Membrane Protein Complexes by Native LDS-PAGE.- Identification of Thioredoxin Target Disulfides Using Isotope-Coded Affinity Tags.- Class III Peroxidases.- Proteotyping of Holm oak (Quercus ilex subsp. ballota) Provenances Through Proteomic Analysis of Acorn Flour.- Immunoproteomics Analysis of Food Allergens.- Beer and Wort Proteomics.- Precipitation of Champagne Base Wine Proteins Prior 2D Electrophoresis.- The Minimal Information About a Proteomics Experiment (MIAPE) from the Proteomics Standards Initiative.
Reihe/Serie | Methods in Molecular Biology ; 1072 |
---|---|
Zusatzinfo | 75 Illustrations, color; 54 Illustrations, black and white; XXI, 786 p. 129 illus., 75 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Botanik | |
Schlagworte | Bioinformatics • Electrophoresis • interactomics • Plant Biology • Proteomics • systems biology |
ISBN-10 | 1-62703-630-X / 162703630X |
ISBN-13 | 978-1-62703-630-6 / 9781627036306 |
Zustand | Neuware |
Haben Sie eine Frage zum Produkt? |
aus dem Bereich