Metabolomics by In Vivo NMR (eBook)

Editors: Robert G. Shulman and Douglas L. Rothman, Yale University School of Medicine, New Haven, Connecticut, USA

Contributors xi

Foreword xiii

Sir George K. Radda, Medical Research Council, UK

Section A: Background

1 Introduction 1

Robert G. Shulman

2 In Vivo NMR Spectroscopy - Techniques; Direct Detection;
MRS; Kinetics and Labels; Fluxes; Concentrations 7

Robin de Graaf

3 Metabolic Control Analysis for the NMR Spectroscopist 31

David A. Fell

Section B: ¯13C and ¯31P NMR Studies of
the Regulation of Glucose Metabolism

4 MRS Studies of the Role of the Muscle Glycogen Synthesis
Pathway in the Pathophysiology of Type 2 Diabetes 45

Gerald I. Shulman and Douglas L. Rothman

5 Phosphorylation of Allosteric Enzymes can serve Homeostasis
rather than Control Flux: the Example of Glycogen Synthase 59

James R.A. Schafer, David A. Fell, Douglas Rothman and Robert G.
Shulman

6 Regulation of Glycogen Metabolism in Muscle during Exercise
73

Thomas B. Price

7 ¯13C NMR Studies of Heart Glycogen Metabolism
87

Maren R. Laughlin, Douglas L. Rothman and Robert G.
Shulman

8 Bioenergetics Implication of Metabolic Fluctuation during
Muscle Contraction 103

Thomas Jue

9 Lactate, Glycogen and Fatigue 125

Robert G. Shulman and Douglas L. Rothman

10 Futile Cycling in Yeast: How to Control Gluttony in the Midst
of Plenty 137

Jan den Hollander and Robert G. Shulman

11 Trehalose Energetics in Yeast Spores 149

Robert G. Shulman and Jan den Hollander

12 Metabolic Networks in the Liver by ²H and
¯13C NMR 159

A. Dean Sherry and Craig R. Malloy

13 Summarized Reflections on Metabolism 175

Robert G. Shulman and James R. A. Schafer

Index 185