Fluorescent Methods to Study Biological Membranes (eBook)
XIV, 486 Seiten
Springer Berlin (Verlag)
978-3-642-33128-2 (ISBN)
Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.
Part I Fluorescence Probes and Model Membranes 1 Laurdan Fluorescence Properties in Membranes : A Journey from the Fluorimeter to the MicroscopeL.A. BAGATOLLI 2 Application of NBD-labeled Lipids in Membrane and Cell Biology S. HALDAR and A. CHATTOPADHYAY 3 3-Hydroxychromone Probes Precisely Located and Oriented in Lipid Bilayers: a Toolkit for Biomembrane ResearchA.S. KLYMCHENKO, G. DUPORTAIL and Y. MÉLY 4 Lateral Membrane Heterogeneity Probed by FRET Spectroscopy and MicroscopyL.M.S. LOURA and M. PRIETO 5 FRET Analysis of Protein-Lipid InteractionsG. GORBENKO and P.K.J. KINNUNEN 6 Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence ShiftsŠ. POKORNA, A. OLŻYŃSKA, P. JURKIEWICZ* and M. HOFMd. JAFURULLA and A. CHATTOPADHYAY Part II Exploring Membrane Organization, Dynamics and Interactions by Advanced Fluorescence-Based Imaging Techniques 7 Visual Discrimination of Membrane Domains in Live Cells by Wide-Field MicroscopyC.E. BUTLER, G. WHEELER, J. GRAHAM and K.M. TYLER 8 Quantitative Fluorescence Studies of Intracellular Sterol Transport and DistributionD. WÜSTNER, F.W. LUND and L.M. SOLANKO 9 Studying Membrane Properties Using Fluorescence Lifetime Imaging Microscopy (FLIM)M.T. STÖCKL, R. BIZZARRI and V. SUBRAMANIAM 10 Fluorescence Correlation Spectroscopy to Study Membrane Organization and InteractionsM. ZELMAN-FEMIAK, Y. SUBBURAJ and A.J. GARCIA-SÁEZ 11 Deciphering Cell Membrane Organization Based on Lateral Diffusion Measurements by Fluorescence Correlation Spectroscopy at Different Length ScalesV. ROUGER, C. BILLAUDEAU, T. TROMBIK, S. MAILFERT, Y. HAMON, H.-T. HE and D. MARGUET 12 STED-FCS Nanoscopy of Membrane DynamicsC. EGGELING 13 Imaging Molecular Order in Cell Membranes by Polarization Resolved Fluorescence MicroscopyS. BRASSELET, P. FERRAND, A. KRESS, X. WANG, H. RANCHON and A. GASECKA 14 Near-Field Optical Nanoscopy of Biological MembranesT.S. van ZANTEN, C. MANZO and M.F. GARCIA-PARAJO Part III Characterization of Membrane Proteins and Receptors by Advanced Fluorescence-based Imaging Techniques 15 Unveiling Biophysical and Biological Properties of a Hypothetical Membrane receptor by Exploiting recent Imaging AdvancesP. GONNORD and R. VARMA 16 New Fluorescent Strategies Shine Light on the Evolving Concept of GCPR OligomerizationM. COTTET, O. FAKLARIS, E. TRINQUET, J.-P. PIN and T. DURROUX 17 Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dymamics of the Serotonin1A Receptor Md. JAFURULLA and A. CHATTOPADHYAY 18 TNF Receptor Membrane Dynamics Studied with Fluorescence Microscopy and SpectroscopyF. NEUGART, D. WIDERA, B. KALTSCHMIDT, C. KALTSCHMIDT and M. HEILEMANN 19 HIV-1 Gag Directed Assembly of Retroviral Particles Investigated by Quantitative Fluorescence ImagingH. de ROCQUIGNY, H. GACEM, P. DIDIER, J. L. DARLIX and Y. MÉLY
Erscheint lt. Verlag | 10.10.2012 |
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Reihe/Serie | Springer Series on Fluorescence | Springer Series on Fluorescence |
Zusatzinfo | XIV, 486 p. |
Verlagsort | Berlin |
Sprache | englisch |
Themenwelt | Naturwissenschaften ► Biologie |
Naturwissenschaften ► Chemie ► Physikalische Chemie | |
Technik | |
Schlagworte | advanced fluorescence microscopy • lipid domains • membrane organization and dynamics • membrane probes • Membrane Proteins • membrane receptors |
ISBN-10 | 3-642-33128-9 / 3642331289 |
ISBN-13 | 978-3-642-33128-2 / 9783642331282 |
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