Nanobioelectrochemistry (eBook)

Dr. Frank N. Crespilho is PhD in Physical-Chemistry and Professor at Institute of Chemistry of São Carlos (IQSC), University of São Paulo (USP), Brazil. He is the author of several works covering the branch of electrochemistry, nanoscience and bioelectrochemistry. Research interests in the Crespilho group are in the areas of special materials and electrochemistry with emphasis on nanomaterials and the bio-nano interface and devices.

Nanobioelectrochemistry 2
Preface 4
1 Nanoscience-Based Electrochemical Sensors and Arrays for Detection of Cancer Biomarker Proteins 8
Abstract 8
1.1…Introduction 9
1.2…Biomarker Proteins and Cancer 9
1.3…Nanomaterials in Protein Sensing Devices 10
1.3.1 Nanomaterials in Electrochemical Immunoassays 10
1.3.2 Nanoparticles as Labels in Immunoassays 13
1.3.3 Coupling Nanostructured Surfaces with Multilabel Enzyme Detection 15
1.3.4 Coupling Nanostructured Surfaces with Electrochemiluminescence (ECL) 20
1.4…Nanostructured Protein Sensors in Microfluidic Arrays 23
1.5…Conclusions and Future Perspectives 26
Acknowledgments 28
References 28
2 Nanomaterials for Biosensors and Implantable Biodevices 34
Abstract 34
2.1…Introduction 35
2.2…Nanostructured Thin Films for Biosensing 37
2.2.1 Langmuir--Blodgett and Layer-by-Layer Based Biosensors 38
2.3…Nanostructured Materials for Biosensing Devices 43
2.3.1 Nanoparticles-Based Biosensors 43
2.3.2 Carbon Materials-Based Biosensors 44
2.4…Miniaturized Devices and Implantable Biosensors 48
2.5…Conclusion 50
References 51
3 Nanomaterials for Enzyme Biofuel Cells 56
Abstract 56
3.1…Introduction 56
3.1.1 Principles of Biofuel Cell Functioning: Mediated or Direct Electron Transfer 58
3.1.2 Characterization of Biofuel Cell Performances 60
3.2…Carbon Nanotube-Based Enzymatic Biofuel Cells 61
3.2.1 Carbon Nanotubes for 3D Electrodes 61
3.2.2 Carbon Nanotubes for Direct Electron Transfer 63
3.2.3 Other Carbon-Based Nanomaterials 65
3.3…Nanoparticle-Based Enzymatic Biofuel Cells 66
3.3.1 Clay Nanoparticles 66
3.3.2 Metal Nanoparticles 69
3.3.3 Other Nanomaterials 70
3.4…Conclusion 70
References 71
4 Biosensors Based on Field-Effect Devices 74
Abstract 74
4.1…Introduction 75
4.2…Field-Effect Devices 76
4.3…Types of Field-Effect Devices 79
4.3.1 Ion-Selective Field-Effect Transistor (ISFET) 79
4.3.2 Electrolyte-Insulator-Semiconductor (EIS) 80
4.3.3 Light-Addressable Potentiometric Sensor (LAPS) 82
4.3.4 Extended-Gate Field-Effect Transistor (EGFET) 83
4.3.5 Separative Extended-Gate Field-Effect Transistor (SEGFET) 84
4.4…Recent Trends Using Field-Effect Sensors 85
4.5…Final Remarks 88
Acknowledgments 88
References 88
5 Using Supramolecular Chemistry Strategy for Mapping Electrochemical Phenomena on the Nanoscale 94
Abstract 94
5.1…General Overview 96
5.2…Construction of Nanoplatforms and Supramolecular Electrochemistry from Functional Electrodes 97
5.3…Nanobiological Sensors as a Natural Inspiration 105
5.4…Concluding Remarks 107
Acknowledgments 107
References 107
6 DNA and Enzyme-Based Electrochemical Biosensors: Electrochemistry and AFM Surface Characterization 112
Abstract 112
6.1…Introduction 113
6.2…DNA-Electrochemical Biosensors 114
6.2.1 AFM Surface Characterization 114
6.2.2 Electrochemistry 116
6.2.3 Applications of DNA-Electrochemical Biosensors 118
6.3…Electrochemical Enzyme Biosensors 120
6.3.1 Carbon Electrode Substrates for Enzyme Biosensors 121
6.3.2 Redox Mediator-Modified Electrodes 122
6.3.3 Enzyme Immobilisation Strategies for Biosensors 123
6.3.4 Direct Electron Transfer and Layer-by-Layer Self-Assembly 125
6.4…Conclusion 128
Acknowledgments 128
References 128
7 Electrochemical-Surface Plasmon Resonance: Concept and Bioanalytical Applications 133
Abstract 133
7.1…Introduction 134
7.2…Surface Plasmon Resonance 135
7.3…Kinetic Parameters 135
7.4…Surface Plasmon Resonance and Electrochemistry 137
7.5…Bioanalytical Applications 138
7.6…Conclusion 141
References 141
Contents 7