Steroid-Protein Interactions II

I. Steroid-Protein Interactions Revisited.- II. Serum Albumin.- A. The Steroid Binding Site of BSA and HSA.- B. Relation of Fatty Acid to Steroid Binding.- C. Steroid Binding to Oligomers of HSA.- D. Characteristics of Steroid Binding to Albumin.- E. Spin Label Studies.- III. ?1-Acid Glycoprotein (AAG, Orosomucoid).- A. Isolation of AAG.- B. Studies on AAG Synthesis.- C. Chemical Properties.- D. Molecular Properties.- E. Interaction with Steroids.- IV. Some Recent Methodological Developments.- 1. Measurement of Unbound Steroid.- 2. Adsorption and Related Methods.- 3. Electrophoretic Methods.- 4. Equilibrium Dialysis, Scatchard Plots and Related Procedures.- V. Corticosteroid-Binding Globulin (CBG).- A. Biosynthesis.- B. Hormonal and Other Influences on Blood CBG Level.- C. General Characteristics of CBG in Nonmammalian Vertebrates.- D. General Characteristics of CBG in Nonhuman Mammals.- E. CBG-Like Steroid Binders.- F. Biological Characteristics of CBG in Human Blood.- G. Physicochemical Properties of Rabbit CBG.- H. Physicochemical and Binding Properties of Guinea Pig CBG.- I. Physicochemical and Binding Properties of Human CBG.- VI. Progesterone-Binding Globulin (PBG).- A. Initial Studies.- B. Occurrence.- C. Origin.- D. Isolation and Physicochemical Properties.- E. Interaction with Steroids.- F. Binding Specificity.- VII. Sex Steroid-Binding Protein (SBP).- A. Introduction.- B. Significance.- C. Methods to Determine SBP Concentration; Data for Human Blood.- D. SPB in Nonmammalian Vertebrates.- E. SBP in Nonhuman Mammals.- F. Occurrence and Development of SBP in Humans.- G. Influence of Endocrines on SBP.- H. SBP in Pathological Conditions.- I. Physicochemical Properties of Human SBP.- J. Steroid Binding to Human SBP.- K. The Binding Site of Human SBP.- VIII.Hydrophobicity and Hydrophilicity of Steroid Binding Sites.- 1. Affinity Constants and Free Energy of Binding.- 2. Dissociation Rates.- 3. Hydrophobicity.- 4. Specificity of Binding; Additivity of ??G°.- 5. The Steroid Binding Site.- IX. Androgen-Binding Protein (ABP).- A. Introduction.- B. ABP in the Rat.- C. ABP in the Rabbit.- D. ABP in Ram, Goat, and Bull.- E. ABP in Man.- F. Possible Function of ABP.- X. Prostatic Steroid-Binding Protein (PSB).- A. Early Observations.- B. General Characteristics of Rat PSB and Its Regulation.- C. Physicochemical and Steroid Binding Properties of Rat PSB.- D. Synthesis of PSB by Translation.- E. Structure of PSB Subunit Components.- F. PSB in Man and Other Species.- XI. Alpha-Fetoprotein.- A. Basic Observations.- B. Purification and Characterization of AFP from Different Species.- C. Binding of Estrogens to AFP.- D. Biological Role of AFP.- XII. Uteroglobin (Blastokinin).- A. Initial Observations.- B. Induction of Uteroglobin.- C. Antibodies Against Uteroglobin.- D. Occurrence of Uteroglobin.- E. Physicochemical Properties of Uteroglobin.- F. Biosynthesis of Uteroglobin.- G. Steroid Binding to Uteroglobin.- H. The Enigmatic Biological Function of Uteroglobin.- XIII. Ecdysteroid-Binding Proteins.- A. Ecdysteroids in the Hemolymph of Insects.- B. Proteins Binding with Low Affinity.- C. High-Affinity Binding.- XIV. Sterol Carrier Protein (SCP).- A. Introduction.- B. SCP in Sterol Biosynthesis.- C. Properties of Sterol Carrier Proteins.- D. SCP-Like Proteins in Adrenal and Other Organs.- E. Additional Cholesterol-Transporting Proteins.- XV. Vitamin D- and Secosteroid Hormone-Binding Serum Proteins.- A. Early Observations.- B. Competitive Binding Analysis Using Vitamin D-Binding Proteins.- C. Determination of Vitamin D-BindingProtein (DBP).- D. General Aspects of DBP in Various Species.- E. Human DBP.- F. Characterization of DBP in Other Vertebrates.- G. DBP-Actin Complex.- H. Hepatic Origin of DBP.- I. Vitamin D Receptors.- J. Physiological Significance of DBP.- XVI. Antibodies to Steroid Hormones.- A. General Aspects.- B. Antibodies Against Digitalis Glycosides and Contraceptive Steroids.- C. Antibodies Against Estrogens.- D. Antibodies Against Androgens.- E. Antibodies Against Progestogens.- F. Antibodies Against Corticosteroids.- G. Physiological Effects of Steroid Antibodies.- XVII. Binding of Steroids to Transforming Enzymes.- A. ?5-3-Ketosteroid Isomerase.- B. 3-Hydroxysteroid Oxidoreductases.- C. Estradiol 17?-Dehydrogenase from Human Placenta and Other Sources.- D. Glutamic Dehydrogenase.- E. 20?-Hydroxy steroid Dehydrogenase from Streptomyces hydrogenans.- A. List of Abbreviations and Symbols.- B. Greek Symbols.- C. Names of Steroids and Other Compounds Used in the Monograph.- D. Adsorption of Steroids to Rubber and Plastics.