Advanced Techniques in Biological Electron Microscopy II

Ultrastructural Localization of Lectin Receptors.- A. Introduction.- B. Purification of Lectins.- C. Purification of Markers.- D. Synthesis of Probes and Labeling Techniques.- References.- Antibody-labeling Techniques.- A. Rationale.- B. Antibody Labels.- C. Methods for Coupling Label to Antibody.- D. Iodination of Antibody.- E. Hemocyanin Label of Antibody.- F. Reaction of Antibody with Cells.- G. Clotting Procedure for Handling Single Cell Suspensions.- H. Radioautography.- I. Replica Techniques.- J. Conclusions.- References.- Cell Surface Labeling for the Scanning Electron Microscope.- A. Introduction.- B. Labeling Techniques for the SEM.- C. Interpretation of Cell Surface Labeling in the SEM.- D. Summary.- References.- Low-Temperature Biological Scanning Electron Microscopy.- A. Introduction.- B. Low-Temperature Solidification of Cell and Tissue Fluids.- C. Pre-treatment Before the Cooling Process.- D. Specimen Cooling.- E. Post-freezing Preparative Procedures.- F. Specimen Transfer.- G. Low-temperature Specimen Stages.- H. Specimen Examination.- I. Conclusions.- References.- Quantitative Electron Microscopy of Nucleic Acids.- A. Introduction.- B. Basic Protein Film Method.- C. Heteroduplex Molecules.- D. Measuring and Error Analysis.- E. Artifacts and Topology Considerations.- F. RNA and Transcription Complexes.- G. Tagging Methods.- F. General Comments and Problems.- H. Protein-free Spreading.- References.- Electron Microscopy of Specific Proteins: Three-Dimensional Mapping of Ribosomal Proteins Using Antibody Labels.- A. Introduction.- B. Techniques.- C Interpretation.- References.- Electron Microscopy and Electron Diffraction Studies on Hydrated Membranes.- A. Introduction.- B. Operation of Hydration Chamber in an Electron Microscope.- C. Electron Microscopy.- D. Electron Diffraction (ED).- E. Conclusions and Future Development.- References.