Trace Elements in Laboratory Rodents

Ronald R. Watson

General Overview: AIN-93 Purified Diets for the Study of Trace Elements Metabolism in Rodents. Basic Tissue Preparation for Electron Microscopy Assessment of Rodents. Iron: Dietary Iron: Deficiency or Excess. The Use of Iron-Dextran to Produce Iron Overload in Rodents. Extraction and Analysis of Iron Species in Diet and Gut. Manganese: Manganese Deficiency and Excess in Rodents. Manganese Uptake in Tissues In Vitro: Tissue Slices as Models. Selenium: Selenium Diets: Deficiency and Excess. Selenium in Tissue Culture. Copper: Copper Deficient and Excess Diets: Theoretical Considerations and Preparations. Copper in Tissue Culture. Zinc: Zinc Diets: Deficiency and Excess. Zinc Radiotracer in the Study of the Mechanisms of Zinc Homeostasis. Other Trace Elements: Dietary Boron Deficiency and Supplementation. Measurements of Boron in Rodent Diets and Tissues. Methods for Studying Dietary Lead and its Toxicity in Rodents. Methods in Chromium Dietary Supplementation and Deficiency. Isolation and In Vitro Analysis of Biologically Active Chromium. Calcium Bioavailability Using a Rat Model. Vanadium Quantitation, Essentiality, and Pharmacologic and Toxicological Studies in Rodents. Nickel-Low Diet Formulation and Tissue Nickel Measurement. Index.