Mitochondrial Bioenergetics (eBook)
XI, 333 Seiten
Humana Press (Verlag)
978-1-61779-382-0 (ISBN)
Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age. These initiatives have fostered the investigation of genetic and environmental influences on bioenergetics. In Mitochondrial Bioenergetics: Methods and Protocols, researchers in the field detail the practical principles and assays designed to derive quantitative assessment of each set of parameters that reflect different aspects of mitochondrial bioenergetics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls
Authoritative and practical, Mitochondrial Bioenergetics: Methods and Protocols helps elevate the quality and rate of investigative discoveries regarding disease states associated with environmental or genetic influences on mitochondrial bioenergetics.
1 - Overview of Mitochondrial Bioenergetics (Vitor M. C. Madeira)
2 – Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells (Ana M. Silva and Paulo J. Oliveira)
3 – High-Resolution Respirometry. OXPHOS Protocols for Human Cells and Permeabilized Fibres from Small Biopsies of Human Muscle (Dominik Pesta and Erich Gnaiger)
4 – High Throughput Analysis of Mitochondrial Oxygen Consumption (James Hynes, Rachel L. Swiss and Yvonne Will)
5 – Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices(Ana Ledo, Rui M. Barbosa and João Laranjinha)
6 - Mitochondrial Membrane Potential (DY) Fluctuations Associated with the Metabolic States of Mitochondria (Carlos M. Palmeira and Anabela P. Rolo)
7 - Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells (Tiago R. Figueira, Daniela R. Melo, Aníbal E. Vercesi and Roger F. Castilho)
8 – Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells (D. Nicholls)
9 – Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria (Vilmante Borutaite and Rasa Baniene)
10 – Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of their Protein Function (Martin Jastroch)
11 - Measurement of Proton Leak and Electron Leak in Isolated Mitochondria
(Charles Affourtit, Casey L. Quinlan and Martin D. Brand)
12 - Relation between Mitochondrial Membrane Potential and ROS Formation (Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski and Mariusz R. Wieckowski)
13 - Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport (António J. Moreno and Joaquim A. F. Vicente)
14 - Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy (Sean M. Davidson and Michael R. Duchen)
15 – Mitochondrial Permeability Transition Pore and Calcium Handling
(Renee Wong, Charles Steenbergen and Elizabeth Murphy)
16- Imaging of Mitochondrial pH Using SNARF-1
(Venkat K. Ramshesh and John J. Lemasters)
17- Redox Equivalents and Mitochondrial Bioenergetics
(James R. Roede, Young-Mi Go and Dean P. Jones)
18-NMR Methodologies for Studying Mitochondrial Bioenergetics
(Tiago C. Alves, Ivana Jarak and Rui A. Carvalho)
19-Computational Modeling of Mitochondrial Function
(Sonia Cortassa and Miguel A. Aon)
| Erscheint lt. Verlag | 6.11.2011 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology |
| Verlagsort | Totowa |
| Sprache | englisch |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik | |
| Schlagworte | bioenergetic performance • drug disturbances • environmental chemical-induced disturbances • environmental influences • genetic influences • hereditary deficiencies • mitochondrial bioenergetics • mitochondrial function |
| ISBN-10 | 1-61779-382-5 / 1617793825 |
| ISBN-13 | 978-1-61779-382-0 / 9781617793820 |
| Haben Sie eine Frage zum Produkt? |
PDF (Adobe DRM)Kopierschutz: Adobe-DRM
Adobe-DRM ist ein Kopierschutz, der das eBook vor Mißbrauch schützen soll. Dabei wird das eBook bereits beim Download auf Ihre persönliche Adobe-ID autorisiert. Lesen können Sie das eBook dann nur auf den Geräten, welche ebenfalls auf Ihre Adobe-ID registriert sind.
Details zum Adobe-DRM
Dateiformat: PDF (Portable Document Format)
Mit einem festen Seitenlayout eignet sich die PDF besonders für Fachbücher mit Spalten, Tabellen und Abbildungen. Eine PDF kann auf fast allen Geräten angezeigt werden, ist aber für kleine Displays (Smartphone, eReader) nur eingeschränkt geeignet.
Systemvoraussetzungen:
PC/Mac: Mit einem PC oder Mac können Sie dieses eBook lesen. Sie benötigen eine
eReader: Dieses eBook kann mit (fast) allen eBook-Readern gelesen werden. Mit dem amazon-Kindle ist es aber nicht kompatibel.
Smartphone/Tablet: Egal ob Apple oder Android, dieses eBook können Sie lesen. Sie benötigen eine
Geräteliste und zusätzliche Hinweise
Buying eBooks from abroad
For tax law reasons we can sell eBooks just within Germany and Switzerland. Regrettably we cannot fulfill eBook-orders from other countries.
aus dem Bereich
