Therapeutic Proteins

Therapeutic Proteins.- Synthetic Antibody Libraries.- The Construction of ‘Phylomer’ Peptide Libraries as a Rich Source of Potent Inhibitors of Protein/Protein Interactions.- Ribosome Display and Screening for Protein Therapeutics.- Yeast Display of Engineered Antibody Domains.- Design, Expression, Purification, and Characterization of Engineered Antibody CH2 and VH Domains.- Engineering of Affibody Molecules for Therapy and Diagnostics.- Protein Design for Diversity of Sequences and Conformations Using Dead-End Elimination.- Design and Generation of DVD-IgTM Molecules for Dual-Specific Targeting.- Engineering and Expression of Bibody and Tribody Constructs in Mammalian Cells and in the Yeast Pichia pastoris.- Use of E. coli for the Production of a Single Protein.- Folding Engineering Strategies for Efficient Membrane Protein Production in E. coli.- Transient Expression Technologies: Past, Present, and Future.- Stable Transfection Pools for Large Quantity of Protein Production.- Mammalian Stable Expression of Biotherapeutics.- Transgenic Expression of Therapeutic Proteins in Arabidopsis thaliana Seed.- Methods for Chromatographic Removal of Endotoxin.- Effectiveness of Various Processing Steps for Viral Clearance of Therapeutic Proteins: Database Analyses of Commonly-Used Steps.- High-Throughput Quantitative N-Glycan Analysis of Glycoproteins.- High-Throughput Multimodal Strong Anion Exchange Purification and N-Glycan Characterization of Endogenous Glycoprotein Expressed in Glycoengineered Pichia pastoris.- Databases and Tools in Glycobiology.- Characterization of PEGylated Biopharmaceutical Products by LC/MS and LC/MS/MS.- Identification of Asp Isomerization in Proteins by 18O Labeling and Tandem Mass Spectrometry.- Monitoring of Subvisible Particles in Therapeutic Proteins.- Size-Exclusion Chromatography with Multi-Angle Light Scattering (SEC-MALS) for Elucidating Protein Aggregation Mechanisms.-Computational Methods to Predict Therapeutic Protein Aggregation.- Coarse-Grained Simulations of Protein Aggregation.- Chitosan-Based Nanoparticles as Delivery Systems of Therapeutic Proteins.- Challenges in the Development and Manufacturing of Antibody-Drug Conjugates.