Vaccinia Virus and Poxvirology

Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections.-In-Fusion® Cloning with Vaccinia Virus DNA Polymerase.-Genetic Manipulation of Poxviruses using Bacterial Artificial Chromosome Recombineering.-Easy and Efficient Protocols for Working with Recombinant Vaccinia Virus MVA.-Isolation of Recombinant MVA using F13L Selection.-Screening for Vaccinia Virus Egress Inhibitors - Separation of IMV, IEV, and EEV.-Imaging of Vaccinia Virus Entry into HeLa Cells.-New Method for the Assessment of Molluscum Contagiosum Virus Infectivity.-An Intradermal Model for Vaccinia Virus Pathogenesis in Mice.-Measurements of Vaccinia Virus Dissemination using Whole Body Imaging: Approaches for Predicting of Lethality in Challenge Models and Testing of Vaccines and Anti-viral Treatments.-Mousepox, a Small Animal Model of Smallpox.-Analyzing CD8 T Cells in Mouse Models of Poxvirus Infection.-Generation and Characterization of Monoclonal Antibodies Specific for Vaccinia Virus.-Bioinformatics for Analysis of Poxvirus Genomes.-Antigen Presentation Assays to Investigate Uncharacterized Immunoregulatory Genes.-Characterization of Poxvirus Encoded Proteins that Regulate Innate Immune Signaling Pathways.-Application of Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) Technology for Studying Interactions of Poxviral Proteins with their Ligands.-Central Nervous System Distribution of the Poxviral Proteins After Intranasal Administration of Proteins and Titering of Vaccinia Virus in the Brain after Intracranial Administration.