Guidelines for Molecular Analysis in Archive Tissues (eBook)

Giorgio Stanta is Professor of Pathology at the University of Trieste. For many years his main interest has been the application of molecular analysis to fixed and paraffin-embedded tissues (all human tissues of biopsy or surgical origin), called “archive tissues”. This research line started thanks to his collaboration with the researchers who first applied molecular biology techniques to human diseases at Yale University, where Professor Stanta worked from 1985 to 1988. Since then he has developed several methods, in particular for RNA analysis. Professor Stanta is the coordinator of the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS" (www.impactsnetwork.eu), involving around 20 European universities in 11 different countries. He is taking part as an expert in the Biobanking and Biomolecular Resources Research European Infrastructure (BBMRI). He is also on the Managing Board of the "Molecular Pathology Study Group" of the European Society of Pathology.

Introduction.- Archive Tissues.- Pre-Analytical Treatment of Tissues.- Dissection of Tissue Components.- Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids.- Reverse Transcription (RT).- Nucleic Acid Amplification.- DNA Sequencing.- Microsatellite Analysis.- Methylation Analysis.- Analysis of Copy Number Variations.- Microarrays.- Protein Extraction in Archive Tissues.- Protein Electrophoretic Separation.- Protein Immunodetection.- Mass Spectrometry.- Elements of Good Laboratory Practice..- DNA Sequencing.- Microsatellite Analysis.- Methylation Analysis.- Analysis of Copy Number Variations.- Microarrays.- Protein Extraction in Archive Tissues.- Protein Electrophoretic Separation.- Protein Immunodetection.- Mass Spectrometry.- Elements of Good Laboratory Practice.