Human Stem Cell Manual (eBook)
488 Seiten
Elsevier Science (Verlag)
978-0-08-054988-0 (ISBN)
Stem cells are self-replicating and undifferentiated, meaning their function is not yet cell, tissue, or organ-specific. Due to the unique nature of these cells, research into their biology and function holds great promise for therapeutic applications through replacement or repair of diseased and damaged cells. This reader-friendly manual provides a practical "e;hands on"e; guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses.
Cover 1
TOC$Contents 6
Foreword by James Battey 10
Foreword by Jeannie Fontana 12
Preface by Jeanne Loring 14
Preface by Robin L. Wesselschmidt 16
Preface by Philip H. Schwartz 18
List of Contributors 20
Part I: Basic Methods in Stem Cell Culture 26
CH$Chapter 1 Human Embryonic Stem Cell Culture 28
CH$Chapter 2 Human Feeder Cells, Feeder-free, and Defined Culture Systems 43
CH$Chapter 3 Mouse Embryonic Fibroblast Feeder Cells 59
CH$Chapter 4 Cryopreservation of Human Embryonic Stem Cells 72
Part II: Characterization of Stem Cells 82
CH$Chapter 5 Classical Cytogenetics: Karyotyping 84
CH$Chapter 6 Spectral Karyotyping and Fluorescent in situ Hybridization 96
CH$Chapter 7 Genotype and Epigenotype by Single Nucleotide Polymorphism (SNP) Analysis 110
CH$Chapter 8 Flow Cytometric Analysis of Human Embryonic Stem Cells 121
CH$Chapter 9 Immunocytochemical Analysis of Stem Cells 133
CH$Chapter 10 Characterization of Stem Cells Using Reverse Transcriptase Polymerase Chain Reaction 152
CH$Chapter 11 Gene Expression Profiling of Stem Cells by Microarray 174
CH$Chapter 12 Generation of Human Embryonic Stem Cell-derived Teratomas 187
CH$Chapter 13 Characterization of Human Embryonic Stem Cell-derived Teratomas 196
Part III: Differentiation of Human Embryonic Cells 208
CH$Chapter 14 Embryoid Bodies and Neuroepithelial Development 210
CH$Chapter 15 Motor Neuron and Dopamine Neuron Differentiation 224
CH$Chapter 16 Oligodendrocyte Differentiation from Human Embryonic Stem Cells 235
CH$Chapter 17 Cardiac Development of Human Embryonic Stem Cells 252
CH$Chapter 18 Hematopoiesis from Human Embryonic Stem Cells 263
Part IV: Genetic Manipulation of Stem Cells 278
CH$Chapter 19 Genetic Manipulation of Human Embryonic Stem Cells: Lentivirus Vectors 280
CH$Chapter 20 Genetic Manipulation of Embryonic Stem Cells 292
Part V: Advanced Methods 314
CH$Chapter 21 Derivation of Embryonic Stem Cells from Human Blastocysts 316
CH$Chapter 22 Neural Progenitor Cell Culture 334
CH$Chapter 23 Stem Cell Transplantation in the Brain 357
CH$Chapter 24 In Vitro Fertilization 376
CH$Chapter 25 Functional Characterization of Neurons 398
Part VI: Setting up a Laboratory 412
CH$Chapter 26 Setting up a Facility for Human Embryonic Stem Cell Research 414
Part VII: Stem Cells and Society 440
CH$Chapter 27 Intellectual Property: Owning the Stem Cell 442
CH$Chapter 28 Ethical Concerns for Stem Cell Research 451
CH$Chapter 29 Guidelines for Embryonic Stem Cell Research Oversight (ESCRO) Committees 462
Abbreviations 474
IDX$Index 478
A 478
B 478
C 478
D 479
E 479
F 480
G 480
H 481
I 481
K 482
L 482
M 482
N 483
O 483
P 483
Q 483
R 484
S 484
T 485
U 486
V 486
W 486
X 486
Y 486
| Erscheint lt. Verlag | 10.10.2011 |
|---|---|
| Sprache | englisch |
| Themenwelt | Medizin / Pharmazie ► Allgemeines / Lexika |
| Studium ► 1. Studienabschnitt (Vorklinik) ► Biochemie / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 0-08-054988-8 / 0080549888 |
| ISBN-13 | 978-0-08-054988-0 / 9780080549880 |
| Haben Sie eine Frage zum Produkt? |
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