Handbook of Immunohistochemistry and in Situ Hybridization of Human Carcinomas (eBook)

Cover 1
Contents 8
Authors and Coauthors of Volume 1 12
Foreword 16
Preface to Volume 1 18
Preface to Volumes 2 and 3 20
Contents of Volumes 2 and 3 22
Introduction to Volumes 2 and 3 26
Prologue 28
Selected Definitions 30
Classification Scheme of Human Cancers 34
Lung and Breast Carcinomas 36
I. Introduction 38
1.1 Comparison of Immunohistochemistry, in situ Hybridization, Fluorescence in situ Hybridization, and Chromogenic in situ Hybridization 40
1.2 Comparison of Chromogenic in situ Hybridization, Fluorescence in situ Hybridization, and Immunohistochemistry 50
1.3 Target and Signal Amplification to Increase the Sensitivity of in situ Hybridization 64
II. Molecular Pathology 80
2.1 Polymerase Chain Reaction Technology 82
2.2 DNA Microarrays Technology 86
2.3 Tissue Microarrays and Their Modifications in High-throughput Analysis of Clinical Specimens 94
2.4 Gene Expression Profiling Using Laser Microdissection in Cancer Tissues 104
2.5 Differential Display of Gene Expression in Human Carcinomas 112
2.6 Serial Analysis of Gene Expression in Human Diseases 122
III. Lung Carcinoma 136
3.1 Lung Carcinoma: An Introduction 138
3.2 Histopathological Classification and Phenotype of Lung Tumors 142
3.3 Immunohistochemistry and in situ Hybridization of Mucin in Lung Carcinoma 152
3.4 Immunohistochemical Expression of MDM2 in Lung Carcinoma 164
3.5 Immunohistochemical Expression of E2F1 and p14ARF in Lung Carcinoma 170
3.6 Role of Immunohistochemical Expression of Beta-Catenin in Lung Carcinoma 178
3.7 Immunohistochemistry of Laminin-5 in Lung Carcinoma 192
3.8 Role of Immunohistochemical Expression of Caveolin-1 in Lung Carcinoma 200
3.9 Role of Thyroid Transcription Factor-1 in Pulmonary Adenocarcinoma 206
3.10 Role of Global Methylation of DNA in Lung Carcinoma 218
3.11 Immunohistochemical and Molecular Pathology of Angiogenesis in Primary Lung Adenocarcinoma 226
3.12 Immunohistochemistry of Human Leukocyte Antigen Expression in Lung Carcinoma 236
3.13 Immunohistochemistry and in situ Hybridization of Telomerase in Lung Carcinoma 242
3.14 Use of Fluorescence in situ Hybridization in Detection of Lung Cancer Cells 250
3.15 Immunohistochemistry of BCL-2 Gene Expression in Lung Carcinoma 260
IV. Breast Carcinoma 268
4.1 Breast Carcinoma: An Introduction 270
4.2 Expression of Vascular Endothelial Growth Factor Receptor-2/Flk-1/KDR in Breast Carcinoma 286
4.3 HER-2/neu Amplification and Protein Overexpression in Breast Carcinoma: Immunohistochemistry and Fluorescence in situ Hybridization 296
4.4 HER-2/neu Amplification Detected by Fluorescence in situ Hybridization in Cytological Samples from Breast Cancer 304
4.5 Detection of HER-2 Oncogene in Human Breast Carcinoma Using Chromogenic in situ Hybridization 316
4.6 Immunohistochemical Evaluation of Sentinel Lymph Nodes in Breast Carcinoma 326
4.7 CD10 Expression in Normal Breast and Breast Cancer Tissues 336
4.8 Role of Immunohistochemical Expression of AKT Protein in Breast Carcinoma 344
4.9 Expression of Extracellular Matrix Proteins in Breast Cancer 358
4.10 Immunohistochemistry of Adhesion Molecule CEACAM1 Expression in Breast Carcinoma 374
4.11 Role of Cadherins in Breast Cancer 380
4.12 Immunohistochemical Expression of Erythropoietin and Erythropoietin Receptor in Breast Carcinoma 388
4.13 Loss of BRCA1 Gene Expression in Breast Carcinoma 398
4.14 Role of Immunohistochemical Defection of BRCA1 in Breast Cancer 408
4.15 Fluorescence in situ Hybridization of BRCA1 Gene in Breast Carcinoma 422
4.16 Immunohistochemistry of c- myc Expression in Breast Carcinoma 432
4.17 Immunohistochemical Localization of Neuropilin-1 in Human Breast Carcinoma: A Possible Molecular Marker for Diagnosis 446
4.18 Role of the Epidermal Growth Factor Receptor in Breast Carcinoma 452
4.19 Alterations of the Cell Cycle Regulating Proteins in Invasive Breast Cancer: Correlation with Proliferation, Apoptosis, and Clinical Outcome 462
4.20 Immunohistochemistry of Estrogen Receptor Expression in Breast Carcinoma 476
4.21 Immunofluorescence and Immunohistochemical Localization of Progesterone Receptors in Breast Carcinoma 486
4.22 Immunohistochemical Expression of Cytosolic Thymidine Kinase in Patients with Breast Carcinoma 500
4.23 Immunohistochemical Detection of Melanoma Antigen E (MAGE) Expression in Breast Carcinoma 508
4.24 Role of Immunohistochemical Expression of Receptors in Male Breast Carcinoma 514
4.25 Detection of Glycoconjugates in Breast Cancer Cell Lines: Confocal Fluorescence 524
4.26 Role of ETV6-NTRK3 Gene Fusion in Breast Carcinoma 530
4.27 Role of CA6 Protein Expression in Breast Carcinoma 542
4.28 Immunohistochemistry of Effusions 550
4.29 Immunohistochemistry of Needle Cytopunctures of Breast Carcinomas 560
Index 570