Guide to Yeast Genetics and Molecular Cell Biology, Part B (eBook)

Cover 1
Table of Contents 6
Contributors to Volume 350 10
Preface 16
Volume in Series 18
Section I: Basic Techniques 40
Chapter 1. Getting Started with Yeast 42
Chapter 2. How to Set up a Yeast Laboratory 81
Chapter 3. Constructing Yeast Libraries 111
Chapter 4. Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glycol Method 126
Chapter 5. Genetic Transformation of Saccharomyces cerevisiae Mitochondria 136
Chapter 6. LacZ Assays in Yeast 151
Chapter 7. Analysis of Budding Patterns 170
Chapter 8. Uses and Abuses of HO Endonuclease 180
Chapter 9. Assays for Gene Silencing in Yeast 204
Section II: Making Mutants 226
Chapter 10. Classical Mutagenesis Techniques 228
Chapter 11. Introduction of Point Mutations into Cloned Genes 238
Chapter 12. Insertional Mutagenesis: Transposon–Insertion Libraries as Mutagens in Yeast 258
Chapter 13. Tn7-Mediated Mutagenesis of Saccharomyces cerevisiae Genomic DNA in Vitro 269
Chapter 14. Vector Systems for Heterologous Expression of Proteins in Saccharomyces cerevisiae 287
Chapter 15. Cloning-Free Genome Alterations in Saccharomyces cerevisiae Using Adaptamer-Mediated PCR 297
Chapter 16. kar-Mediated Plasmid Transfer between Yeast Strains: Alternative to Traditional Transformation Methods 317
Chapter 17. Gene Disruption 329
Chapter 18. Synthetic Dosage Lethality 355
Section III: Genomics 366
Chapter 19. Saccharomyces Genome Database 368
Chapter 20. Three Yeast Proteome Databases: PombePD, and CalPD (MycoPathPD) 386
Chapter 21. Database Resources Relevant to Yeast Biology 412
Chapter 22. Searching Yeast Intron Data at Ares Lab Web Site 419
Chapter 23. Yeast Genomic Expression Studies Using DNA Microarrays 432
Chapter 24. Transcriptome Analysis of Saccharomyces cerevisiae Using Serial Analysis of Gene Expression 453
Chapter 25. PCR-Based Engineering of Yeast Genome 484
Chapter 26. ChIP-chip: A Genomic Approach for Identifying Transcription Factor Binding Sites 508
Chapter 27. Computational Approaches to Identifying Transcription Factor Binding Sites in Yeast Genome 523
Section IV: Proteomics 536
Chapter 28. Array-Based Methods for Identifying Protein Protein and Protein–Nucleic Acid Interactions 538
Chapter 29. Building Protein–Protein Networks by Two- Hybrid Mating Strategy 552
Chapter 30. Integrated Version of Reverse Two-Hybrid System for the Postproteomic Era 564
Chapter 31. Biochemical Genomics Approach to Map Activities to Genes 585
Chapter 32. Use of Two-Dimensional Gels in Yeast Proteomics 598
Author Index 624
Subject Index 648