Conditional Mutagenesis: An Approach to Disease Models (eBook)

Preface 5
List of Contents 8
List of Contributors 10
Tools for Conditional Mutagenesis 11
Conditional Somatic Mutagenesis in the Mouse Using Site- Specific Recombinases 12
Cre/loxP-Mediated Chromosome Engineering of the Mouse Genome 38
Tetracycline-Controlled Genetic Switches 58
Novel Gene Switches 82
Improved Embryonic Stem Cell Technologies 115
Gene Trap Mutagenesis 137
RNA Interference in Mice 156
Viral Vectors: AWide Range of Choices and High Levels of Service 184
Conditional Mutagenesis by Cell-Permeable Proteins: Potential, Limitations and Prospects 210
Examples of Conditional Disease Models 240
Analysis of Mouse Development with Conditional Mutagenesis 241
Conditional Mouse Models of Cancer 269
Conditional Mutagenesis Reveals Immunological Functions ofWidely Expressed Genes: Activation Thresholds, Homeostatic Mechanisms and Disease Models 294
Conditional Transgenesis and Recombination to Study the Molecular Mechanisms of Brain Plasticity and Memory 320
A Novel Conditional Knockout Strategy Applied to Serotonin Receptors 351
Conditional Mouse Models for Friedreich Ataxia, a Neurodegenerative Disorder Associating Cardiomyopathy 368
Animal Models in Cardiovascular Diseases: New Insights from Conditional Models 379
Conditional Animal Models for the Study of Lipid Metabolism and Lipid Disorders 408
Conditional Mouse Models to Study Developmental and Pathophysiological Gene Function in Muscle 441
Analysis of Calcium Channels by Conditional Mutagenesis 469
Conditional Mutagenesis of G-Protein Coupled Receptors and G- Proteins 491
Contribution of Targeted Conditional Somatic Mutagenesis to Deciphering Retinoid X Receptor Functions and to Generating Mouse Models of Human Diseases 510
Subject Index 524