EMC 2008 (eBook)

Preface Volume 3: Life Science 5
Content 7
Cryoelectron microscopy: from molecules to systems 23
Progress in High-resolution Scanning Probe Microscopy 25
Modular organization of RNase P 27
Molecular anatomy of the human pathogen 29
Unveiling conformational changes of biological molecules using multiscale modeling and multiresolution experiments 31
Nicolas Boisset: In memoriam 33
Cryo TEM-based 3D reconstruction of the recombinant expressed human zinc peptidase Meprin ß 35
Origin melting by SV40 Large T antigen-new insights from 3D- EM- MLF3D classification method 37
Sequence analysis and modelling of the two large subunits of Phosphorylase Kinase 39
Cellular Uptake of Polymer Nanoparticles Imaged by Electron Microscopy 41
9 Å cryo-EM structure and molecular model of a gastropod hemocyanin didecamer ( KLH1) reveals the architecture of the asymmetric collar 43
Microstructure of model systems for sauces based in polysacharides observed by Cryo- SEM 45
Three-dimensional architecture of outer- and inner-dynein arms in flagella revealed by cryo- electron tomography and single particle analysis 47
Structural and functional studies of rabbit skeletal muscle Phosphorylase Kinase 49
Single-particle analysis of the Cdc6/Orc1, archaeal DNA replication initiator protein 51
A high-throughput acquisition toolbox in Matlab for cryo- electron microscopy studies 53
Architecture of bacterial glutamate synthase complexe using hybrid approaches 55
3D Structure of DNA Repair Macromolecular Complexes Participating in Non- Homologous End- Joining ( NHEJ) 57
Quaternary structure of recombinant human meprin a zinc peptidase of the astacin family 59
Allosterism of Nautilus pompilius hemocyanin as deduced from 8 Å cryo-EM structures obtained under oxy and deoxy conditions 61
High resolution structure of a 6 MDa protease by xray- crystallography and cryo- EM 63
Cryo-TEM of liquid crystalline particles: Application of materials science techniques to study liquid crystalline particle structures. 65
Structural basis for the concerted integration of HIV- 1 DNA in the human genome, role of the human cofactor LEDGF/ p75. 67
Titan Krios: Automated 3D imaging at ambient and cryogenic conditions 69
Self assembly and phase behaviour of new sugar based gemini amphiphiles 71
Peptide-nanotube formation by lysine based lipoamino acids 73
Advances in biological microanalysis using event streamed spectrum imaging and programmed beam acquisition 75
3D-correlation-averaging for membrane-protein-crystals 77
8 Å cryo-EM structure of the giant hemoglobin from the planorbid snail Biomphalaria glabrata 79
Structure of Pex5p and Pex5-20 complexes in the yeast Hansenula polymorpha. Pex20p causes a conformational change upon binding to Pex5p tetramers involved in peroxisomal protein transport. 81
Structure of OprM-MexA interacting complex revealed by cryo electron tomography 83
Pleiomorphic viruses revealed by cryo tomography: the structure of coronaviruses 85
Combined structural and chemical analysis of unique anammox bacteria that contain a prokaryotic organelle 87
Structural and functional considerations on the 3- D organization of the fenestrated cytoplasm of hepatic endothelial cells 89
Molecular architecture of the presynaptic compartment studied by cryo- electron tomography 91
Synapses in high pressure frozen Caenorhabditis elegans 93
High resolution EM-tomography of a whole vitrified cell 95
Evaluation of beam damage in catalase crystals observed in vitrified sections 97
Urothelial fusiform vesicles are formed in post- Golgi compartment 99
Localisation of GFP and GFP tagged PKD2 in cultivated pancreatic cancer cells using high- pressure freezing 101
Cytomegalovirus membrane envelopment revisited – a STEM- tomography study based on high- pressure freezing and freeze substitution. 103
Electron tomography & template matching of biological membranes
Correlated Microscopy: From Dynamics to Structure 107
3DEM-analyses of Golgi apparatus subunits during physiologic and pathologic reorganizations 109
Visualization of the 80s ribosome in situ using cryo electron tomography of vitreous sections 111
Three-dimensional analysis of the intermediate filament network using SEM- tomography 113
The apical vesicles of the tuft cells in the main excretory duct of the rat submandibular gland by EFTEM- TEM tomography 115
(S)TEM Dual Axis Tomography 117
STEM tomography of high-pressure frozen cell monolayers 119
Multimodality of pericellular proteolysis in cancer cell invasion 121
Photonic crystal type nanoarchitectures in butterfly wing scales 123
Iridovirus-like viruses of Lacerta monticola from Serra da Estrela, Portugal 125
Structural and ultrastructural investigations on the in vitro effects of plant extracts in chicken and rabbit whole blood cultures 127
Fine structural characterization of cloned human endogenous retrovirus HERV- K113 129
The procaine hydrochlorate effect onto the corpuscular anthocyans from the vacuolar sap of different plant cells 131
Comparative anatomical and ultrastructural investigations on normal and vitroplantlets leaves from Pistia stratiotes L. 133
Autophagy of mitochondria in brown adipocytes of chemically thyroidectomised rats 135
Ultrastructural studies of immune organs in antigen primed chickens treated with vegetal extractions 137
Structural features of HIV-1 immunologic activation 139
Functions of actin-binding proteins in the cell nucleus 141
Ultrastructural mechanisms of entamoebae movement system 143
White adipocytes transdifferentiation into brown adipocytes induced by triiodothyronine 145
Ultrastructural studies of the tegument of cestodes ( Platyhelminthes): phylogenetic implications 147
Crystallization stages of the CaCO3 deposits in the earthworm’s calciferous gland 149
Scanning electron microscopic analysis of heavy metal resistant microorganisms 151
Characterization of mouse embryoid bodies by Scanning Electron Microscopy ( SEM) and Confocal Laser Scanning Microscopy ( CLSM) 153
Stage-dependent localization of mitochondrial DNA during the cell cycle of Euglena gracilis Z by immunogold electron microscopy 155
The virus-inducted structures in RNA virus infected macrophages 157
Surface layers of ore-leaching Bacteria and Archaea 159
Structure of Bacillus anthracis spores analyzed by CEMOVIS 161
Insights into the role of pUL71 during HCMV morphogenesis 163
Fine structure of the genital chamber of female Chrysomya megacephala (F.) (Diptera: Calliphoridae) 165
Ultrastructure of the testes and spermatozoa of the blow fly Chrysomya megacephala (F.) (Diptera:Calliphoridae) 167
Biosynthesis of cellulose in red algae 169
Light and electron microscopy aspects of the glandular sessile hairs from the vitroplantlet leave of Drosera rotundifolia 171
Ultrastructure of selected Euglena species in relation to their taxonomy 173
Confocal Microscopy Reveals Molecular Interactions Between DNA- binding Drugs and Chromatin in Live Cells 175
Fluorescence microscopy methods for measuring the mobility and stability of molecules in 3- D samples 177
Phasor-FLIM analysis of FRET for homotypic and heterotypic non- covalent interactions: stimulated membrane receptors in live cells 179
In vitro immunofluorescence and ultrastructural analysis of the expression of noncollagenous matrix proteins in human pulpal cells induced by mineralizing factors 181
Differentiation-dependent Golgi fragmentation in the bladder urothelial cells in vitro 183
Monitoring Mitochordria Dynamics in Living Cell System by Confocal Microscopy 185
The freeze-fracture replica immunolabeling technique for the study of lipid droplet biogenesis and function 187
Dynamics of epigenome duplication, accessibility and translation 189
Controlled light exposure microscopy reveals telomeric microterritories throughout the cell cycle 191
Calibration in fluorescence correlation spectroscopy for measurements of stem cell differentiation kinetic 193
Spectral methods for cells and particles identifications 195
3D realistic visualization of supramolecular assemblies 197
Heterosis in Arabidopsis thaliana: Structural aspects in mature and germinating seeds from hybrid and parental lines 199
Colour visualization of red blood cells in native smears by the new method reflected light microscopy 201
In vitro culture of Trigonella foenum-graecum plantules and their anatomic characterization 203
Live cell imaging by SEM-hosted X-ray microscope in “ water window” energy range 205
Microscopic anatomy and mineral composition of cuticle in amphibious isopods Ligia italica and Titanethes albus (Crustacea:Isopoda) 207
In vivo imaging and quantification of the continuous keratin filament network turnover 209
Necrotic cell death, a controlled way of cellular explosion 211
Dissecting mitochondrial protein distributions using sub- diffraction resolution fluorescence microscopy 213
High Thoughput, High Content Tissue Cyotometry 215
Focus on the vascular wall: Imaging of large arteries using two- photon microscopy 217
High resolution confocal Ca2+ imaging of the pulmonary neuroepithelial body microenvironment in lung slices 219
Two photon excitation microscopy and SHG imaging as a tool for visualisation of type I and type II collagen, and their use in tissue engineering 221
Testing calibration standards for confocal and two- photon microscopy 223
Distribution of the voltage-gated delayed-rectifier K+ subunits, Kv1.1 and Kv1.2, in the adult murine enteric nervous system 225
Observation of Ventricular Myocyte Morphology in Long Term Culture using High Resolution Confocal Imaging 227
A magnetic field enhances the inmunofluorescence signal in a lymphocytic cell 229
Novel 515 nm DPSS-laser brings excitement in life cell imaging 231
Confocal laser scanning microscopy study on human fissure caries stained with alizarin red 233
Non-invasive skin tissue characterization using non- linear spectral imaging microscopy 235
Force Spectroscopy reveals mechanical structure of capsids 237
Manipulating and imaging molecular motors with optical traps, single- molecule fluorescence and atomic force microscopy 239
Mechanical stability of ethanol-induced A-DNA 241
Manipulation of the mechanical properties of a virus by protein engineering 243
A single-molecule mechanical assay to study DNA replication coupled to DNA unwinding 245
Generation of multiple DNA lesions at subnuclear resolution by multi- photon irradiation 247
Light and electron microscopy of phagosomes 249
The Use of Electron Microscopy in the Diagnosis of Human Microsporidial Infection – the Manchester, UK, Experience 251
Overview of the impact of biological microanalysis in health and disease 253
Immunolocalization of FOXP3 in HCV-infected liver biopsies. Preliminary observations. 255
Ultrastructural and Intracellular Elemental Composition Analysis of Human Hematopoietic Cells During Cold Storage in Preservation Solutions 257
Reconstitution and morphological characterisation of an original Human Endogenous Retrovirus 259
Cell death in human articular chondrocyte: an ultrastructural study in micromass model. 261
Pathomorphological diagnostic of paraffin embedded versus epon embedded cardiac tissues with transmission electron microscope analysis 263
Interaction of carbon nanotubes with macrophages: STEM and EELS approach 265
The ultastructural effect of nitrogen mustard gas on rat brain and therapeutic value of proanthocyanidin 267
Assembly and maturation of pestiviruses 269
Analysis of radiation-induced bystander effects using high content screening 271
Electron microscopic investigations on normal and dexamethasone applied rat placentas 273
Changes in dental enamel birefringence after CO2 laser irradiation through fluoride gel – a pilot study 275
SEM and light microscopic examination and morphometric analysis of temporal changes in intimal and medial connective tissue component of human carotid arteries 277
Ultrastructure of human amniotic membrane covering villous chorion and smooth chorion in preeclampsia 279
Changes in Intracellular Sodium, Chlorine, and Potassium Content in Hematopoietic Cells after Hypotermic Storage 281
Requirements for a bright future of electron microscopy in the rapid laboratory diagnosis of infectious diseases 283
Changed thickness of zona pellucida as a result of stimulated ovulation 285
Mozaic microscopy of pancreatic islets 287
Microscope history Database 289
Interaction of H-Ras Transformed Cell Line with Folic Acid Modified Magnetic Nanoparticles and Detection by Transmission Electron Microscope 291
Sedimentation of suspensions for diagnostic thin section electron microscopy 293
Evaluation of rofecoxib administration influence on ultrastructural image of the kidney 295
Microstructural changes produced by Pulsed Electric Fields in liquid whole egg studied by Transmission Electron Microscopy ( TEM) 297
Microstructural changes in dough treated by Glucose oxidase ( GOX) and Transglutaminase ( TG), studied by Scanning Electron Microscopy ( SEM) 299
Hyperspectral imaging – a novel concept for marker free chromosome characterization 303
Poxviruses: Morphogenesis of an Orthopoxvirus (CPXV) and an Avipoxvirus ( FPXV) in host tissue 305
Ultrastructural alterations of methotrexate in mouse kidney 307
Ultrastructural gadolinium evidence in skin of a patient with Nephrogenic Fibrosing Dermopathy 309
Nephrotoxicity induced by inorganic Hg(II) and Pb(II): a microscopic and biochemical in vitro study 311
Tissue distribution of peroxisomes in zebrafish 313
An analysis of the ultrastructure of matured HIV 315
Ultrastructural analysis of lysosomal storage diseases: effects of therapy 319
Immunolabeling for Electronmicroscopy 321
Molecular Organisation of Cadherins in Intercellular Adhesion Junctions by Cryo- Electron Tomography of Vitreous Sections 323
Ultrastructural observations of indium in the lactating mammary gland cells 325
An active mechanism flanks and modulates the export of the small ribosomal subunits 327
New methods for micro-domain detection in bacterial sacculi 329
Subcellular localization of Myogenic Regulatory Factors along skeletal muscle development 331
Immunocytochemical Strategies: LR Resins or Lowicryl, Gold or Peroxidase – Which is Better? 333
Elemental Analysis in Electron Microscopy for Medical Diagnostics 335
Synaptic localization of KCa1.1 potassium channels in central neurons revealed by postembedding immunogold and SDS- digested freeze- fracture replica labelling 337
Ultrastructural studies of rod photoreceptor nuclei from SCA7 mouse 339
Application of electron energy loss spectroscopy for heavy metal localization in unicellular algae 341
Localization of “flagella” proteins in the Archaeon Ignicoccus hospitalis 343
Detection of gold nanoparticles by Autrata YAG detector in FE SEM working in cryo mode 345
Correlative cryo-fluorescence and electron microscopy 347
The effect of hypometabolizing molecules on transcription as shown by a new two- step pulse- chase method 349
Cryofixation and Freeze-Substitution Combined with Tokuyasu Cryo- section Labelling 351
The Drosophila active zone architecture: combining confocal, STED and transmission electron microscopy 353
Distribution and function of amorphous CaCO3 and calcite within the tergite cuticle of terrestrial isopods (Crustacea) 355
A symbiosis: tracking cell signaling with expression probes, quantum dots and a programmable array microscope ( PAM) 357
Golgi twins in mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy 359
Understanding the 3D architecture of organelle bound protein complexes using cryo- electron tomography of frozen hydrated sections and immunogold our next great challenge
Cryo-electron microscopy of vitreous sections 363
The visualization of viruses in the low- voltage electron microscope 365
Exploring HPF/FS methods for sensitive immunogold labeling on resin sections 367
Low contrast of the ER-membranes in the high pressure frozen, freeze substituted specimens 369
HPF of cultured cell monolayers: towards a standard method for high pressure freezing, freeze substitution and electron tomography acquisition 371
Life-like physical fixation of large samples for correlative microscopy 373
Integrating automated single, double and triple in situ molecular detection with imaging for the analysis of inter- and intracellular events in the seminiferous tubule 375
Freeze-substitution in Epon: An attempt to combine immunolabeling and improvement of structural preservation 377
The Shape of Caveolae is Omega-like after Chemical Fixation and Cup- like after Cryofixation 379
Integrated laser and transmission electron microscopy 381
Correlative 3D microscopy: CLSM and FIB/SEM tomography used to study cellular entry of vaccinia virus 383
Strategies for the morphological analysis of hydrated and life- like preserved biomedical material in the SEM 385
Denaturation of metaphase chromatin plates observed by transmission electron microscopy and atomic force microscopy in aqueous solution 387
Discovery of a Nuclear Structure in UVC-induced Apoptotic Cells by Integrated Laser Electron Microscopy 389
Analysis of biomineral formation in three-dimensional micro- mass stem cell cultures 391
Comparative structural studies of highly-filled polymethacrylates with light microscopy, SEM, TEM and AFM 393
Cell surface characteristics as reporters for cellular energy state 395
Analysis of multimodal 3D microscopy measurements 397
Permanent plastid – nuclear complexes (PNCs) in plant cells 399
Cell imaging by dynamic secondary ion mass spectrometry ( SIMS): basic principles and biological applications 401
Water imaging in cells cryosections by electron energy loss spectroscopy 403
Study of the cellular uptake of Pt nanoparticles in human colon carcinoma cells 405
Ion spectrometry and electron microscopy correlative imaging for accurate comprehensive trace element localization at subcellular level, in normal and pathological keratinocytes 407
Calcium Spark Detection and Analysis in Time Series of Two- Dimensional Confocal Images 409
Visualisation of the attachment, possible uptake and distribution of technical nanoparticles in cells with electron microscopy methods 411
Author Index 413
Subject Index 423