Two Hybrid Technologies
Humana Press Inc. (Verlag)
978-1-61779-454-4 (ISBN)
Authoritative and accessible, Two Hybrid Technologies: Methods and Protocols supplies researchers with a comprehensive toolbox for the identification of biologically relevant protein interactions.
Matrix-Based Yeast Two-Hybrid Screen Strategies and Comparison of Systems.- Array-Based Yeast Two-Hybrid Screens: A Practical Guide.- High Throughput Yeast Two-Hybrid Screening.- A Stringent Yeast Two-Hybrid Matrix Screening Approach for Protein-Protein Interaction Discovery.- High-Throughput Yeast Two-Hybrid Screening of Complex cDNA Libraries.- Virus-Human Cell Interactomes.- Interactome Mapping in Malaria Parasites: Challenges and Opportunities.- Mapping Interactomes with High Coverage and Efficiency Using the Shifted Transversal Design.- Assigning Confidence Scores for Protein-Protein Interactions.- The Intergration and Annotation of the Human Interactome in the UniHI Database.- Gene-Centered Yeast One-Hybrid Assays.- One- plus Two-hybrid System for the Efficient Selection of Missense Mutant Alleles Defective in Protein-Protein Interactions.- Investigation of Membrane Protein Interactions Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System.- Application of the Split-Protein Sensor Trp1 to Protein Interaction Discovery in the Yeast Saccharomyces cerevisiae.- Tetracycline Repressor-Based Mammalian Two-Hybrid Systems.- The Fluorescent Two-Hybrid (F2H) Assay for Direct Analysis of Protein-Protein Interactions in Living Cells.- ArrayMAPPIT: a Screening Platform for Human Protein Interactome Analysis.- MAPPIT as a High-Throughput Screening Assay for Modulators of Protein-Protein Interactions in HIV and HCV.- Integrated Measurement of Split TEV and cis-Regulatory Assays Using EXT Encoded Reporter Libraries.
Reihe/Serie | Methods in Molecular Biology ; 812 |
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Zusatzinfo | XI, 329 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | interactomics • protein-protein interaction detection methods • virus-human cell interactomes • yeast two-hybrid approach • yeast two-hybrid high-throughput screens |
ISBN-10 | 1-61779-454-6 / 1617794546 |
ISBN-13 | 978-1-61779-454-4 / 9781617794544 |
Zustand | Neuware |
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