cDNA Libraries

Isolation of Fungal Infection Structures from Plant Tissue by Flow Cytometry for Cell-Specific Transcriptome Analysis.- Preparation of a High Quality cDNA Library from a Single-Cell Quantity of mRNA Using Chum-RNA.- Construction of a Full-Length cDNA Library from Castor Endosperm for High-throughput Functional Screening.- Full-Length Transcriptome Analysis Using a Bias-Free cDNA Library Prepared with the Vector-Capping Method.- Construction of Improved Yeast Two-Hybrid Libraries.- Normalization of Full-Length Enriched cDNA.- Bioinformatic Methods for Finding Differentially Expressed Genes in cDNA Libraries, Applied to the Identification of Tumour Vascular Targets.- Enzymatic Production of RNAi Libraries from cDNAs and High-Throughput Selection of Effective shRNA Expression Constructs.- Construction of Small RNA cDNA Libraries for High-Throughput Sequencing.- Focusing Mutations Within Random Libraries to Distinct Areas: Protein Domain Library Generation by Overlap Extension (PDLGO).- Generation of Families of Construct Variants Using Golden Gate Shuffling.- Application of Full-Length cDNA Resources to Gain-of-Function Technology for Characterization of Plant Gene Function.- Construction of Yeast Surface-Displayed cDNA Libraries.- Identification of Protein/Target Molecule Interactions Using Yeast Surface-Displayed cDNA Libraries.- SNP Discovery by Transcriptome Pyrosequencing.- RNA-seq Analysis of Gene Expression and Alternative Splicing by Double-Random Priming Strategy.- Generation of a Large Catalog of Unique Transcripts for Whole-Genome Expression Analysis in Non-Model Species.