Enzyme Histochemistry - Z. Lojda, R. Gossrau, T.H. Schiebler

Enzyme Histochemistry

A Laboratory Manual
Buch | Softcover
X, 344 Seiten
1979
Springer Berlin (Verlag)
978-3-540-09269-8 (ISBN)
106,99 inkl. MwSt
During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

A. Introduction.- B. General Considerations.- I. Conditions for the Histochemical Demonstration of Enzymes.- II. Terminology and Classification of Enzymes.- III. Principles of Reactions in Histochemical Methods for the Detection of Enzymes.- IV. Artifacts and Control Reactions.- V. Correlation of Histochemical and Biochemical Findings.- C. Preparation of Tissues.- I. Tissue Sampling.- II. Further Treatment.- III. Incubation.- D. Detection Methods.- I. Instructions.- II. Hydrolases.- III. Transferases.- IV. Lyases.- V. Oxidoreductases.- E. Solutions and Buffers.- F. List of Firms.- G. References.- H. Subject Index and Source List.

Erscheint lt. Verlag 1.6.1979
Zusatzinfo X, 344 p. 116 illus.
Verlagsort Berlin
Sprache englisch
Maße 170 x 244 mm
Gewicht 670 g
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete
Studium 2. Studienabschnitt (Klinik) Pathologie
Naturwissenschaften Biologie Biochemie
Schlagworte Amino acid • Coenzym • Disaccharid • Enzyme • enzymes • Fructose • Glutamat • Glycogen
ISBN-10 3-540-09269-2 / 3540092692
ISBN-13 978-3-540-09269-8 / 9783540092698
Zustand Neuware
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