Arthritis Research
Humana Press Inc. (Verlag)
978-1-61737-507-1 (ISBN)
In Volume 1, authors discuss synovial joint morphology, histopathology, and immunohistochemistry, cartilage matrix and bone biology, and cell trafficking, migration and invasion. Volume 2 is broken up into sections that cover immunobiology and T cells, animal models of arthritis, and applications of new technologies, such as Differential Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR), to define novel therapeutic targets. Both volumes combine to produce a concise set of protocols condensing decades of experience and expertise.
Arthritis Research: Methods and Protocols will be a valuable tool for basic research investigators, clinician scientists, pathologists, immunologists, and biochemists looking to stay current in their fields.
Synovial Joint Morphology, Histopathology, and Immunohistochemistry.- Imaging Inflamed Synovial Joints.- Arthroscopy as a Research Tool.- Immunohistochemistry of the Inflamed Synovium.- In Situ Hybridization of Synovial Tissue.- Subtractive Hybridization.- Laser Capture as a Tool for Analysis of Gene Expression in Inflamed Synovium.- Preparation of Mononuclear Cells from Synovial Tissue.- Quantitative Image Analysis of Synovial Tissue.- Cartilage Matrix and Bone Biology.- Cartilage Histomorphometry.- Image Analysis of Aggrecan Degradation in Articular Cartilage With Formalin-Fixed Samples.- In situ Detection of Cell Death in Articular Cartilage.- Measurement of Glycosaminoglycan Release from Cartilage Explants.- Assessment of Collagenase Activity in Cartilage.- Assessment of Gelatinase Expression and Activity in Articular Cartilage.- Analyses of MT1-MMP Activity in Cells.- Analysis of TIMP Expression and Activity.- Bone Histomorphometry in Arthritis Models.- Generation of Osteoclasts In Vitro, and Assay of Osteoclast Activity.- Cell Trafficking, Migration, and Invasion.- Isolation and Analysis of Large and Small Vessel Endothelial Cells.- Analysis of Flow-Based Adhesion In Vitro.- Analysis of Leukocyte Recruitment in Synovial Microcirculation by Intravital Microscopy.- Angiogenesis in Arthritis.- Analysis of Inflammatory Leukocyte and Endothelial Chemotactic Activity.- Acquisition, Culture, and Phenotyping of Synovial Fibroblasts.- Genotyping of Synovial Fibroblasts.- Gene Transfer to Synovial Fibroblast.- In Vitro Matrigel Fibroblast Invasion Assay.- Culture and Analysis of Circulating Fibrocytes.
Erscheint lt. Verlag | 10.11.2010 |
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Reihe/Serie | Methods in Molecular Medicine ; 135 |
Zusatzinfo | 15 Illustrations, color; 99 Illustrations, black and white; XX, 446 p. 114 illus., 15 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 152 x 229 mm |
Themenwelt | Medizinische Fachgebiete ► Chirurgie ► Unfallchirurgie / Orthopädie |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 1-61737-507-1 / 1617375071 |
ISBN-13 | 978-1-61737-507-1 / 9781617375071 |
Zustand | Neuware |
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