Exocytosis and Endocytosis

An Overview of Methods for Analyzing Vesicular Trafficking.- Vesicular Trafficking: Molecular Tools and Targets.- Pharmacological Inhibition of Endocytic Pathways: Is It Specific Enough to Be Useful?.- Cell-Free and Biochemical Assays of Exocytosis and Endocytosis.- In Vitro Assays to Measure SNARE-Mediated Vesicle Fusion.- Imaging Ca2+-Triggered Exocytosis of Single Secretory Granules on Plasma Membrane Lawns from Neuroendocrine Cells.- FRAP Analysis of Secretory Granule Lipids and Proteins in the Sea Urchin Egg.- A Cell-Free Assay for Endocytosis of E-Cadherin.- Cell-Surface Biotinylation to Study Endocytosis and Recycling of Occludin.- Fractionation of Subcellular Membrane Vesicles of Epithelial and Nonepithelial Cells by OptiPrep™ Density Gradient Ultracentrifugation.- Identification and Characterization of Interacting Partners of Rab GTPases by Yeast Two-Hybrid Analyses.- Analysis of Endocytosis and Exocytosis in Different Cell Systems.- High-Throughput Analysis of the Dynamics of Recycling Cell Surface Proteins.- Studying Phagocytosis by Live-Cell Scintillation Proximity Assay.- Transcytosis of Polymeric Immunoglobulin A in Polarized Madin–Darby Canine Kidney Cells.- The Use of Syntaxin Chimeras to Study Polarized Protein Trafficking in Epithelial Cells.- Targeting the Epithelial SNARE Machinery by Bacterial Neurotoxins.- Exocytosis of Endothelial Cells Is Regulated by N-Ethylmaleimide-Sensitive Factor.- Molecular Dissection of HCl Secretion in Gastric Parietal Cells Using Streptolysin O Permeabilization.- Addressing Membrane Protein Topology Using the Fluorescence Protease Protection (FPP) Assay.- Visualizing Clathrin-Mediated IgE Receptor Internalization by Electron and Atomic Force Microscopy.- Analyzing Endosomes in Nonsectioned Cells by TransmissionElectron Microscopy.- Imaging Exocytosis of Single Insulin Secretory Granules With TIRF Microscopy.- Reconstitution of Depolarization and Ca2+-Evoked Secretion in Xenopus Oocytes Monitored by Membrane Capacitance.- Low-Noise Recording of Single-Vesicle Capacitance Steps in Cell-Attached Patches.- Good Practices in Single-Cell Amperometry.- Analysis of Single-Vesicle Exocytotic Events Recorded by Amperometry.- Analysis of Exocytosis and Endocytosis in Model Organisms.- Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in Caenorhabditis elegans.- FM 1-43 Labeling of Synaptic Vesicle Pools at the Drosophila Neuromuscular Junction.- Probing E-Cadherin Endocytosis by Morpholino-Mediated Rab5 Knockdown in Zebrafish.- Quantifying Endocytosis In Vivo Using Intravital Two-Photon Microscopy.