Laser Capture Microdissection -

Laser Capture Microdissection

Methods and Protocols
Buch | Softcover
334 Seiten
2010
Humana Press Inc. (Verlag)
978-1-61737-458-6 (ISBN)
102,67 inkl. MwSt
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Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip(R) microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI(R) approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.

Part I. Introduction An Introduction to Laser-Based Tissue Microdissection Techniques Stephanie Curran and Graeme I. Murray Part II. Microdissection and DNA Analysis Methacarn Fixation for Genomic DNA Analysis in Microdissected Cells Makoto Shibutani and Chikako Uneyama Multiplex Quantitative Real-Time PCR of Laser Microdissected Tissue Patrick H. Rooney Comparative Genomic Hybridization Using DNA From Laser Capture Microdissected Tissue Grace Callagy, Lucy Jackson, and Carlos Caldas Detection of Ki-ras and p53 Mutations by Laser Capture Microdissection/PCR/SSCP Deborah Dillon, Karl Zheng, Brina Negin, and Jose Costa Whole-Genome Allelotyping Using Laser Microdissected Tissue Colleen M. Feltmate and Samuel C. Mok Microdissection for Detecting Genetic Aberrations in Early and Advanced Human Urinary Bladder Cancer Arndt Hartmann, Robert Stoehr, Peter J. Wild, Wolfgang Dietmaier, and Ruth Knuechel Laser Microdissection for Microsatellite Analysis in Colon and Breast Cancer Peter J. Wild, Robert Stoehr, Ruth Knuechel, Arndt Hartmann, and Wolfgang Dietmaier Assessment of RET/PTC Oncogene Activation in Thyroid Nodules Utilizing Laser Microdissection Followed by Nested RT-PCR Giovanni Tallini and Guilherme Brandao Combined Laser-Assisted Microdissection and Short Tandem Repeat Analysis for Detection of In Situ Microchimerism After Solid Organ Transplantation Ulrich Lehmann, Anne Versmold, and Hans Kreipe Part III. RNA and Gene Expression Studies Using Microdissected Cells, Laser-Assisted Microdissection of Membrane-Mounted Tissue Sections Lise Mette Gjerdrum and Stephen Hamilton-Dutoit. Laser-Assisted Microdissection of Membrane-Mounted Sections Following Immunohistochemistry and In Situ Hybridization Lise Mette Gjerdrum and StephenHamilton-Dutoit Laser-Assisted Cell Microdissection Using the PALM System Patrick Micke, Arne Ostman, Joakim Lundeberg, and Fredrik Ponten Laser Microdissection and RNA Analysis Ludger Fink and Rainer Maria Bohle Gene Expression Profiling of Primary Tumor Cell Populations Using Laser Capture Microdissection, RNA Transcript Amplification, and GeneChip(R) Microarrays Veronica I. Luzzi, Victoria Holtschlag, and Mark A. Watson Quantification of Gene Expression in Mouse and Human Renal Proximal Tubules Jun-ya Kaimori, Masaru Takenaka, and Kousaku Okubo Laser Capture Microdissection for Analysis of Macrophage Gene Expression From Atherosclerotic Lesions Eugene Trogan and Edward A. Fisher Analysis of Pituitary Cells by Laser Capture Microdissection Ricardo V. Lloyd, Xiang Qian, Long Jin, Katharina Ruebel, Jill Bayliss, Shuya Zhang, and Ikuo Kobayaski Part IV. Microdissection Techniques and Applications in Proteomics Laser Capture Microdissection and Colorectal Cancer Proteomics Laura C. Lawrie and Stephanie Curran Proteomic Analysis of Human Bladder Tissue Using SELDI(R) Approach Following Microdissection Techniques Rene C. Krieg, Nadine T. Gaisa, Cloud P. Paweletz, and Ruth Knuechel Part V. Microdissection and Molecular Analysis of Microorganisms Genetic Analysis of HIV by In Situ PCR-Directed Laser Capture Microscopy of Infected Cells Daniele Marras Use of Laser Capture Microdissection Together With In Situ Hybridization and Real-Time PCR to Study the Distribution of Latent Herpes Simplex Virus Genomes in Mouse Trigeminal Ganglion Xiao-Ping Chen, Marina Mata, and David J. Fink Laser Capture Microdissection and PCR for Analysis of Human Papilloma Virus Infection Kheng Chew, Patrick H. Rooney, Margaret E. Cruickshank, and Graeme I. Murray Laser Capture Microdissection

Erscheint lt. Verlag 9.12.2010
Reihe/Serie Methods in Molecular Biology ; 293
Zusatzinfo 81 black & white illustrations, 1 colour illustrations, biography
Verlagsort Totowa, NJ
Sprache englisch
Maße 229 x 152 mm
Gewicht 495 g
Einbandart Paperback
Themenwelt Naturwissenschaften Biologie Biochemie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
Naturwissenschaften Biologie Zellbiologie
ISBN-10 1-61737-458-X / 161737458X
ISBN-13 978-1-61737-458-6 / 9781617374586
Zustand Neuware
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