Gene Delivery to Mammalian Cells

Volume 2: Viral Gene Transfer Techniques

Buch | Softcover

566 Seiten

2010 | Softcover reprint of hardcover 1st ed. 2004
Humana Press Inc. (Verlag)
978-1-61737-353-4 (ISBN)

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The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.

Delivery Using Adenoviruses.- Adenovirus-Mediated Gene Delivery.- DNA Delivery to Cells in Culture.- Adenovirus-Mediated Gene Delivery to Skeletal Muscle.- Delivery of Adenoviral DNA to Mouse Liver.- Delivery of DNA to Lung Airway Epithelium.- Delivery of DNA to Pulmonary Endothelium Using Adenoviral Vectors.- Gene Transfer to Brain and Spinal Cord Using Recombinant Adenoviral Vectors.- Adenovirus-Mediated Gene Transfer to Tumor Cells.- Adenovirus-Mediated Gene Delivery to Dendritic Cells.- Delivery Using Adeno-Associated Viruses.- Overview of Adeno-Associated Viral Vectors.- AAV Vector Delivery to Cells in Culture.- AAV-Mediated Gene Transfer to Skeletal Muscle.- AAV-Mediated Gene Transfer to the Liver.- AAV-Mediated Gene Transfer to Mouse Lungs.- Gene Delivery to the Mammalian Heart Using AAV Vectors.- Gene Delivery to the Mouse Brain with Adeno-Associated Virus.- Delivery of DNA to Tumor Cells In Vivo Using Adeno-Associated Virus.- Gene Delivery to Human and Murine Primitive Hematopoietic Stem and Progenitor Cells by AAV2 Vectors.- Delivery Using Herpes Simplex Viruses.- Delivery Using Herpes Simplex Virus.- Gene Transfer to Skeletal Muscle Using Herpes Simplex Virus-Based Vectors.- Delivery of Herpes Simplex Virus-Based Vectors to the Nervous System.- Gene Transfer to Glial Tumors Using Herpes Simplex Virus.- Delivery of Herpes Simplex Virus-Based Vectors to Stem Cells.- Delivery Using Baculoviruses.- Baculovirus-Mediated Gene Delivery into Mammalian Cells.- Delivery Using Lentiviruses.- Gene Delivery by Lentivirus Vectors.- Lentiviral Vectors for the Delivery of DNA into Mammalian Cells.- Stable Gene Delivery to CNS Cells Using Lentiviral Vectors.- Gene Delivery to Hematopoietic Stem Cells Using Lentiviral Vectors.- Delivery of Genes to the Eye Using Lentiviral Vectors.- Lentiviral Transduction of Human Dendritic Cells.- Delivery Using Retrovirus.- Gene Transfer by Retroviral Vectors.- Gene Delivery to Cells in Culture Using Retroviruses.- Retrovirus-Mediated Gene Transfer to Tumors.- Dilivery of Genes to Hematopoietic Stem Cells.- Delivery Using Alphaviruses.- Delivery and Expression of Heterologous Genes in Mammalian Cells Using Self-Replicating Alphavirus Vectors.

Erscheint lt. Verlag	10.11.2010
Reihe/Serie	Methods in Molecular Biology ; 246
Zusatzinfo	XVIII, 566 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-61737-353-2 / 1617373532
ISBN-13	978-1-61737-353-4 / 9781617373534
Zustand	Neuware