Generation of cDNA Libraries

Complementary DNA Libraries.- Rapid Amplification of cDNA Ends.- cDNA Generation on Paramagnetic Beads.- Construction of a Normalized cDNA Library by mRN-cDNA Hybridization and Subtraction.- Amplification of cDNA Ends Using PCR Suppression Effect and Step-Out PCR.- Use of Inverse PCR to Clone cDNA Ends.- Construction of Size-Fractionated cDNA Library Assisted by an In Vitro Recombination Reaction.- Construction of a Full-Length Enriched and a 5?-End Enriched cDNA Library Using the Oligo-Capping Method.- cDNA Library Construction Using In Vitro Transcriptional Amplification.- Amplification of Representative cDNA Pools from Microscopic Amounts of Animal Tissue.- Single-Cell cDNA Library Construction Using Cycling aRNA Amplification.- mRNA/cDNA Library Construction Using RNA—Polymerase Cycling Reaction.- Quality Assessment of cDNA Libraries.- Assessment of the Quality of mRNA Libraries by Agarose Gel Electrophoresis.- PACS RT-PCR.- Single-Cell mRNA Library Analysis by Northern Blot Hybridization.- Generation of cDNA Libraries for Profiling Gene Expression of Given Tissues or Cells.- Screening Poly [dA/dT(?)] cDNA for Gene Identification.- Generation of Longer cDNA Fragments from SAGE Tags for Gene Identification.- Generation of Full-Length cDNA Libraries Enriched for Differentially Expressed Genes.- Subtractive Hybridization for the Identification of Differentially Expressed Genes Using Uraci-DNA Glycosylase and Mung-Bean Nuclease.- Subtractive Cloning of Differential Genes Using RNA-PCR.- Strategy for Construction of a cDNA Encoding a Repetitive Amino Acid Sequence.- Preparing Lambda Libraries for Expression of Proteins in Prokaryotes or Eukaryotes.- Peptide Library Construction from RNA-PCR-Derived RNAs.- Identifying Interacting Proteins in an Escherichiacoli-Based Two-Hybrid System.- Future Perspectives.

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