Matrix Metalloproteinase Protocols

Part I. MMPs and TIMPs-An Overview of the Field. MMPs and TIMPs: An Historical Perspective, J. Frederick Woessner, Jr. Strategies for Cloning New MMPs and TIMPs, Gloria Velasco and Carlos Lopez-Otin. Structural Studies of MMPs and TIMPs, Wolfram Bode and Klaus Maskos. Matrix Metalloproteinase Substrate Binding Domains, Modules and Exosites: Overview and Experimental Strategies, Christopher M. Overall. The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes: Transcriptional and Posttranscriptional Regulation, Signal Transduction and Cell-Type-Specific Expression, Matthew P. Vincenti. Models for Gain-of-Function and Loss-of-Function of MMPs: Transgenic and Gene Targeted Mice, Lisa M. Coussens, Steven D. Shapiro, Paul D. Soloway, and Zena Werb. Part II. Expression and Purification of MMPs and TIMPs. Expression of MMPs and TIMPs in Mammalian Cells, Karen M.-L. Yeow, Blaine W. Phillips, P. Paul Beaudry, Kevin J. Leco, Gillian Murphy, and Dylan R. Edwards. Expression of Recombinant Matrix Metalloproteinases in Escherichia coli, L. Jack Windsor and Darin L. Steele. Expression of Human Collagenase I (MMP-1) and TIMP-1 in a Baculovirus-Based Expression System, Rudiger Vallon and Peter Angel. Expression of Recombinant Matrix Metalloproteinases in Yeast, Glenn A. Doyle. Expression of Recombinant Membrane-Type MMPs, Michael J. Butler, Marie-Pia d'Ortho, and Susan J. Atkinson. Refolding of TIMP-2 from Escherichia coli Inclusion Bodies, Richard A. Williamson. Expression and Refolding of Full-Length Human TIMP-1, Deborah Davis. Purification of MMPs and TIMPs, Ken-ichi Shimokawa and Hideaki Nagase. Part III. Detection of MMPs and TIMPs. Monitoring MMP and TIMP mRNA Expression by RT-PCR, Howard Wong, Huong Muzik, Lori L. Groft, Marc A. Lafleur, Charles Matouk, Peter A. Forsyth, Gilbert A. Schultz, Steven J. Wall, and Dylan R. Edwards. Measuring Transcription of Metalloproteinase Genes: Nuclear Run-Off Assay vs Analysis of hnRNA, Anne M. Delany. In Situ Hybridization for Metalloproteinases and Their Inhibitors, Tina L. Hurskainen and Suneel S. Apte. Use of EIA to Measure MMPs and TIMPs, Noboru Fujimoto and Kazushi Iwata. Immunohistochemistry of MMPs and TIMPs, Yasunori Okada. Detecting Polymorphisms in MMP Genes, Shu Ye and Adriano M. Henney. Part IV. Assay of MMP and TIMP Activities. Methods for Studying Activation of Matrix Metalloproteinases, Vera Knauper and Gillian Murphy. Assay of Matrix Metalloproteinases Against Matrix Substrates, Timothy E. Cawston, Paul Koshy, and Andrew D. Rowan. Zymography and Reverse Zymography for Detecting MMPs and TIMPs, Susan P. Hawkes, Hongxia Li, and Gary T. Taniguchi. In Situ Zymography, Sarah J. George and Jason L. Johnson. Detection of Focal Proteolysis Using Texas-Red-Gelatin, Rosalind M. Hembry. Antibodies to MMP-Cleaved Aggrecan, Amanda J. Fosang, Karena Last, David C. Jackson, and Lorena Brown. Cartilage Proteoglycan Release Assay, Caron J. Billington. Immunoassay for Collagenase-Mediated Cleavage of Types I and II Collagens, R. Clark Billinghurst, Mirela Ionescu, and A. Robin Poole. Collagen Degradation Assays, Anthony P. Hollander. Invasion Assays and Matrix Metalloproteinases: Quantification of Cellular Invasion Using Propidium Iodide Labeling and Confocal Laser Scanning Microscopy, Ulrike Benbow, Kenneth A. Orndorff, and Alice L. Givan. Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases, Gregg B. Fields. Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloproteinases (TIMP), Mike Hutton and Frances Willenbrock. Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases, Kyoko Yamashita and Taro Hayakawa. Index.