Nuclease Methods and Protocols

Characterizing Nuclease Activity.- Processivity of DNA Repair Enzymes.- Analysis by HPLC of Distributive Activities and the Synthetic (Back) Reaction of Pancreatic-Type Ribonucleases.- Ier1 p: A Kinase and Site-Specific Endoribonuclease.- Microtiter-Plate Assay and Related Assays for Nonspecific Endonucleases.- Quantitating mRNAs with Relative and Competitive RT-PCR.- Expressing Self-Incompatibility RNases (S-RNases) in Transgenic Plants.- Molecular Cloning, Tissue Distribution, and Chromosomal Localization of the Human Homolog of the R2/Th/Stylar Ribonuclease Gene Family.- Inhibitors and Activators of Nucleases.- Ribonuclease Inhibitors.- Producing Soluble Recombinant RNases and Assays to Measure Their Interaction with Interferon-? In Vitro?.- Assays for the Evaluation of HIV-1 Integrase Inhibitors.- The Direction of Ribonucleases H by Antisense Oligodeoxynucleotides.- The 2–5A/RNase L Pathway and Inhibition by RNase L Inhibitor (RLI).- Relating Nucleas Structure and Function.- Crystallization and Crystal Structure Determination of Ribonuclease A-Ribonuclease Inhibitor Protein Complex.- Methods for Studying the Interaction of Barnase with Its Inhibitor Barstar.- Assaying In Vitro Refolding of RNases by Mass Spectrometry.- Dissecting Nucleases into Their Structural and Functional Domains.- Using Electrostatics to Define the Active Site of Serratia Endonuclease.- Homology Modeling and Simulations of Nuclease Structures.- Methods for Determining Activity and Specificity of DNA Binding and DNA Cleavage by Class II Restriction Endonucleases.- Engineered Properties and Assays for Human DNase I Mutants.- Nucleases in the Clinic.- Assays for Human DNase I Activity in Biological Matrices.- Evaluation of Retroviral Ribonuclease H Activity.- Assays for Detection of RNase ASuperfamily Ribonucleases.- Assay for Antitumor and Lectin Activity in RNase Homologs.- Isolation and Enzymatic Activity of Angiogenin.- Preparation and Preclinical Characterization of RNase-Based Immunofusion Proteins.- Assays Using Nucleases.- Restriction Endonucleases and Their Uses.- Isolation and Characterization of an Unknown Restriction Endonuclease.- Gene Modification with Hapaxoterministic Restriction Enzymes.- Techniques to Measure Nucleic Acid-Protein Binding and Specificity.- Analyzing the Developmental Expression of Sigma Factors with S1-Nuclease Mapping.- Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays.