Lectin Methods and Protocols

Lectin Histochemistry and Cytochemistry.- Light Microscopy.- Lectin Histochemistry and Cytochemistry—Light Microscopy.- Lectin—Gold Histochemistry on Paraffin and Lowicryl K4M Sections Using Biotin and Digoxigenin-Conjugated Lectins.- Use of Fluorochrome-Labeled Lectins in Light Microscopy.- The Use of Lectins in Combination with Enzymatic Digestion for the Study of Glycoconjugates in Cartilage.- Applications of Lectin Histochemistry and Cytochemistry in Diagnosis and Prognosis.- Electron Microscopy.- Electron Microscopy.- Amplification of Lectin-Gold Histochemistry.- Electron Microscopic Methods for the Demonstration of Lectin-Binding Sites in Cancer Cell Lines.- Use of Lectins for Structural Analysis of Oligosaccharide Chains.- Application of Sequential Smith Degradation to Lectin Blots.- Blot Analysis with Lectins for the Evaluation of Glycoproteins in Cultured Cells and Tissues.- Characterization of HIV gp120 Envelope Glycoprotein by Lectin Analysis.- Use of Lectins for Characterization of O-Linked Glycans of Herpes Simplex Virus Glycoproteins.- Lectins for Detection of Altered Glycosylation of Circulating Glycoproteins.- Use of Lectin for Detection of Agalactosyl IgG.- Lectins for Detection of Altered Glycosylation of Circulating Glycoproteins.- Detection of Altered Glycosylation of ?-Fetoprotein Using Lectin-Affinity Electrophoresis.- Use of Lectin-Affinity Electrophoresis for Quantification and Characterization of Glycoforms of ?-1 Acid Glycoprotein.- ABO(H) Blood Group Expression on Circulating Glycoproteins.- Use of Lectins in Quantification of Soluble Glycoproteins.- Lectin/Antibody “Sandwich” ELISA for Quantification of Circulating Mucin as a Diagnostic Test for Pancreatic Cancer.- Quantification of Intestinal Mucins.- Lectins in AffinityPurification of Soluable Glycoproteins.- Purification and Characterization of Human Serum and Secretory IgA1 and IgA2 Using Jacalin.- Use of Lectins in Affinity Purification of HIV and SIV Envelope Glycoproteins.- T-Cell Receptor Purification.- Lectins in Flow Cytometry.- Use of Monomeric, Monovalent Lectin Derivatives for Flow Cytometric Analysis of Cell Surface Glycoconjugates.- Analysis of Subcellular Components by Fluorescent-Lectin Binding and Flow Cytometry.- Lectins as Tools for Cell Purification/Purging.- Lectins as Tools for the Purification of Liver Endothelial Cells.- The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM) Purging and Hematopoietic Progenitor Cell Enrichment in Clinical Bone Marrow Transplantation.- Combined Lectin/Monoclonal Antibody Purging of Bone Marrow for Use in Conjunction with Autologous Bone Marrow Transplantation in the Treatment of Multiple Myeloma.- Effects of Lectins on Mammalian Cells.- Mechanisms and Assessment of Mitogenesis.- Mitogenic Effects of Lectins on Epithelial Cells.- Use of Lectins as Mitogens for Lymphocytes.- Effect of Lectins on Uptake of Polyamines.- Effects of Lectins on Cytoskeletal Organization in Mammalian Cells.- Effect of Lectins on Protein Kinase Activity.- Lectin-Induced Calcium Mobilization in Human Platelets.- Lectin-Triggered Superoxide/H2O2 and Granule Enzyme Release from Cells.- Cytotoxic Effects of Lectins.- Application of the Lectin-Dependent Cell-Mediated Cytotoxicity Assay to Bronchoalveolar Lavage Fluid and Venous Blood Samples Collected from Canine Lung Allografts.- Effects of Lectins in Organ Culture.- The Effect of Lectins on Crypt Cell Proliferation in Organ Culture.- Effects of Lectin Ingestion.- Effects of Lectin Ingestion on Animal Growth and Internal Organs.- Lectin Ingestion.- Assessment of Lectin Inactivation by Heat and Digestion.- Lectins in The Investigation of Neuronal Trafficking.- Use of Lectins as Transganglionic Neuronal Tracers in the Study of Unmyelinated Primary Sensory Neurons.- Use of Lectins in The Investigation of Pathogen-Host Interactions.- Lectin Inhibition of Bacterial Adhesion to Animal Cells.- Inhibition of HIV Infection by Lectin Binding to CD4.- Inhibition of HIV Infection by Lectin Binding to gp120.- Use of Lectins for Drug Delivery.- Absorption Enhancement by Lectin-Mediated Endo- and Transcytosis.- The Use of Lectins for Liposome Targeting in Drug Delivery.