The Nucleus

The Intranuclear Environment.- The Intranuclear Environment.- Isolation of Nuclei.- Purification of Nuclei and Preparation of Nuclear Envelopes from Skeletal Muscle.- Isolation of Highly Purified Yeast Nuclei for Nuclease Mapping of Chromatin Structure.- Working with Oocyte Nuclei: Cytological Preparations of Active Chromatin and Nuclear Bodies from Amphibian Germinal Vesicles.- Preparation of Arabidopsis Nuclei and Nucleoli.- High-Yield Isolation and Subcellular Proteomic Characterization of Nuclear and Subnuclear Structures from Trypanosomes.- Methods for Studying the Nuclei and Chromosomes of Dinoflagellates.- The Nucleolus.- Isolation of Nucleoli from Ehrlich Ascites Tumor Cells and Dynamics of Nascent RNA within Isolated Nucleoli.- Time-lapse Microscopy and Fluorescence Resonance Energy Transfer to Analyze the Dynamics and Interactions of Nucleolar Proteins in Living Cells.- Three-Dimensional Reconstruction of Nucleolar Components by Electron Microscope Tomography.- Intranuclear Structures.- The Perinucleolar Compartment (PNC): Detection by Immunohistochemistry.- Isolation of the Constitutive Heterochromatin from Mouse Liver Nuclei.- Isolation of Pathology-Associated Intranuclear Inclusions.- The Nuclear Ubiquitin–Proteasome System: Visualization of Proteasomes, Protein Aggregates, and Proteolysis in the Cell Nucleus.- Interphase Chromosomes.- Multicolor 3D Fluorescence In Situ Hybridization for Imaging Interphase Chromosomes.- Fluorescent Transgenes to Study Interphase Chromosomes in Living Plants.- Analysis of Telomeres and Telomerase.- Combined Immunofluorescence, RNA Fluorescent In Situ Hybridization, and DNA Fluorescent In Situ Hybridization to Study Chromatin Changes, Transcriptional Activity, Nuclear Organization, and X-Chromosome Inactivation.- Analysisof the Mobility of DNA Double-Strand Break-Containing Chromosome Domains in Living Mammalian Cells.