Modern Biooxidation – Enzymes, Reactions and Applications

Rolf Schmid obtained his PhD in 1970. After post-doctoral studies in France and the USA, he joined the research laboratories of Henkel KGaA in D?orf in 1972 where he became Director of the Biotechnology Laboratories. In 1987, he moved to the 'Gesellschaft f?technologische Forschung' (GBF) in Braunschweig where he headed the Division of Enzyme Technology and Natural Products Research. In 1993, he accepted an invitation to build up the newly founded Institute for Technical Biochemistry at the University of Stuttgart. He is further in charge of the Department of Molecular Biotechnology at the Fraunhofer Institute for Membrane Science and Biotechnology in Stuttgart, and he is chairman of the IUPAC Commission of Biotechnology. Vlada B. Urlacher obtained her diploma in Biology at the University of Tashkent, Uzbekistan. After receiving her PhD from the Academy of Sciences, Uzbekistan, she spent a postdoctoral year at the Institute of Technical Biochemistry, University of Stuttgart, Germany and then two years in the Institute of Biotechnology, University Halle-Wittenberg, Germany. In 2001 she returned to the Institute of Technical Biochemistry, University of Stuttgart. Since 2003 she has been head of Biocatalysis group at this Insitute. Her main research interests are engineering the technical application of oxidizing enzymes.

1. INTRODUCTION AND HISTORY 2. BIOOXIDATION FOR MEDICAL AND PHARMACEUTICAL RESEARCH 2.1 Preparation of Drug Metabolites Using Fungal and Bacterial Strains 2.2 Liver Monooxygenases 2.3 Recombinant Human Liver Monooxygenases in Pharmacological Research 2.4 Human Cytochrome P450 Monooxygenases - A General Model of Substrate Specificity and Regioselectivity 2.5 Steroid Oxidation 3. BIOOXIDATION FOR BIOCATALYSIS AND ORGANIC SYNTHESIS 3.1 Selective Microbial Oxidations in Industry 3.2 PQQ- and FAD-Dependant Oxidases 3.3 Laccases and their Applications 3.4 Dioxygenases and their Applications 3.5 Heme Monooxygenases: A Superfamily, their Substates and their Applications 3.6 Electroenzymologie with Monooxygenases: Challenges, Perspectives and Practical Considerations 3.7 Baeyer-Villiger Monooyxgenases 3.8 Heme Monooxygenases: The Reductase Issue 3.9 A Modular Approach to Biotransformation Using Microbial Cytochromes 4. PLANT MONOOXYGENASES AND THEIR APPLICATIONS 5. COFACTOR REGENERATION 6. BIOOXIDATION IN INDUSTRY