Enzyme Assays – High–throughput Screening, Genetic Selection and Fingerprinting

1985 Jean Louis Reymond obtained his diploma working with Professor Seebach at the ETH Zurich. He finished his thesis in the field of total synthesis in 1989 in the group of Professor Vogel. After being in the United States for two years at the Scripps Research Institute he became an assistant professor there. 1997 he returned to the University of Bern, Switzerland. His research interest is biocatalysis and in particular enzyme design, assays, profiling, fingerprints and inhibitors.

PART I. THE CHEMISTRY OF ENZYME ASSAYS 1. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity 2. High throughput screening systems for assaying the enantioselectivity of enzymes 3. High throughput screening methods developed for oxidoreductases 4. Industrial perspective on assays PART II. ENZYME ASSAYS AND GENETIC SELECTION 5. Agar plate based assays 6. Genetic selection for biocatalysts 7. Three hybrid systems and in vivo selection 8. Nucleic acid based approaches on the screening of enzymes. PART III. ENZYME PROFILING 9. Fluorescent probes for lipolytic enzymes 10. Enzyme fingerprinting 11. Protease profiling 12. Enzyme assays on chips INDEX