Protein and Peptide Analysis by LC-MS

Thomas Letzel is associate professor for analytical chemistry and bioanalysis at the Wissenschaftszentrum Weihenstephan (WZW) located at the TUM (Technische Universitõt M³nchen), Germany. He obtained his PhD in Chemistry with studies about soot aerosols and environmental analysis at the Chair for Analytical Chemistry of TUM. After a postdoc time in Amsterdam, The Netherlands, at the VU Amsterdam with topics in pharmaceutical and enzymatic analysis, he came back to TUM and was employed as a scientist, leading a new analytical research group with focus on bioanalysis. In latter position he got his Habilitation. He developed and established mass spectrometric techniques coupled with modern liquid phase solution techniques in enzymology, studying noncovalent complexes and observing molecular fingerprints. His research of novel mass spectrometric techniques comprises the identification and characterization of organic molecules in applications of biological, environmental, pharmaceutical, food, plant and nutrition sources.

Sample Preparation;

How to Couple and Handle HPLC and Mass Spectrometry;

LC-MS of Proteins;

LC-MS of Membrane Proteins;

LC-MS of Hydrolyzed Proteins;

Online-digest-LC-MS of Proteins;

Quantitative LC-MS of Proteins;

Top-Down Analysis;

LC-MS for the Identification of posttranslational modifications on proteins;

Functional Analysis of Proteins by LC-MS;

Bioinformatical Tools for the LC-MS Analysis of Proteins;

Industrial Standards and Strategies in LC-MS Analysis of Proteins