Benson's Microbiological Applications Complete Version

Professor and Chair, Consultant Environmental Microbiology

Part 1 Microscopy 1 Brightfield Microscopy 2 Darkfield Microscopy 3 Phase-Contrast Microscopy 4 Fluorescence Microscopy 5 Microscopic Measurements Part 2 Survey of Microorganisms 6 Protozoa, Algae and Cyanobacteria 7 Ubiquity of Bacteria 8 The Fungi: Molds and Yeasts Part 3 Manipulation of Microorganisms 9 Aseptic Technique 10 Pure Culture Techniques Part 4 Staining and Observations of Microorganisms 11 Smear Preparation 12 Simple Staining 13 Negative Staining 14 Capsular Staining 15 Gram Staining 16 Spore Staining: Two Methods 17 Acid-Fast Staining: Kinyoun Method 18 Motility Determination Part 5 Culture Methods 19 Culture Media Preparation 20 Preparation of Stock Cultures 21 Enumeration of Bacteria: The Standard Plate Count 22 Slide Culture: Fungi Part 6 Bacterial Viruses 23 Determination of a Bacteriophage Titer 24 Burst Size Determination: A One-Step Growth Curve 25 Isolation of Phage from Flies 26 Phage Typing Part 7 Environmental Influences and Control of Microbial Growth 27 Effects of Oxygen on Growth 28 Temperature: Effects on Growth 29 pH and Microbial Growth 30 Water Activity and Osmotic Pressure 31 Ultraviolet Light: Lethal Effects 32 The Effects of Lysozyme on Bacterial Cells 33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic 34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method 35 Evaluation of Antiseptics: The Filter Paper Disk Method 36 Effectiveness of Hand Scrubbing Part 8 Identification of Unknown Bacteria 37 Morphological Study of An Unknown Bacterium 38 Cultural Characteristics 39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions 40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions 41 Physiological Characteristics: Multiple Test Media 42 Use of Bergey's Manual Part 9 Miniaturized Multitest Systems 43 Enterobacteriaceae Identification: The API 20E System 44 Enterobacteriaceae Identification: The Enterotube II System 45 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System 46 Staphylococcus Identification: The API Staph System Part 10 Diversity and Environmental Microbiology 47 Isolation of an Antibiotic Producer: The Actinomyces 48 Nitrogen Cycle: Ammonification 49 Symbiotic Nitrogen Fixation: Rhizobium 50 Free-Living Nitrogen Fixation: Azotobacter 51 Denitrification: Paracoccus denitrificans 52 The Winogradsky Column 53 Purple Nonsulfur Photosynthetic Bacteria 54 Sulfate Reducing Bacteria: Desulfovibrio 55 Bacterial Commensalism 56 Bacterial Synergism 57 Microbial Antagonism Part 11 Applied Microbiology 58 Bacterial Food Counts 59 Bacteriological Examination of Water: Most Probable Number Determination 60 Bacteriological Examination of Water: The Membrane Filter Method 61 Reductase Test 62 Temperature: Lethal Effects 63 Microbial Spoilage of Canned Food 64 Microbiology of Alcohol Fermentation Part 12 Bacterial Genetics and Biotechnology 65 Mutant Isolation by Replica Plating 66 Bacterial Transformation 67 Polymerase Chain Reaction for Amplifying DNA 68 Plasmid Isolation Part 13 Medical Microbiology 69 The Staphylococci: Isolation and Identification 70 The Streptococci and Enterococci: Isolation and Identification 71 Gram-Negative Intestinal Pathogens 72 A Synthetic Epidemic Part 14 Immunology and Serology 73 Slide Agglutination Test: Serological Typing 74 Slide Agglutination Test for S. aureus 75 Slide Agglutination Test for Streptococcus 76 Tube Agglutination Test: The Heterophile Antibody Test 77 Blood Grouping Appendices