Chemistry and Biology of Pteridines / Montreal, Canada, June 15–20, 1986

Frontmatter -- Preface / Cooper, Bernard A. / Whitehead, V. Michael -- Acknowledgments -- Contents -- How it all Began: A Brief History of the Pteridine Symposia / Albert, Adrien -- The Gowland Hopkins Lecture: The Mechanisms of Action of Folate and Pterin Requiring Enzymes / Benkovic, S.J. / Slieker, L.J. / Daubner, S.C. / Courtney, L.F. / Dix, T.A. / Pember, S.O. / Bloom, L.M. / Fierke, C.A. / Mayer, R.J. / Chen, J.-T. / Taira, K. -- Section A: Chemistry of Pterins and Folates -- Side-Chain Chemistry of Pteridines / Pfleiderer, W. / Kang, Y. / Soyka, R. / Hutzenlaub, W. / Wiesenfeldt, M. / Leskopf, W. -- A General Method for the Synthesis of 10-Substituted-10-Deaza Folate Analogues / Nair, M.G. / Toghiyani, Tahereh R. / Ramamurthy, B. / Kisliuk, R.L. / Gaumont, Y. -- Synthesis and Properties of Pyrimidodiazepines as Ring-Strained Probes of Pterin Utilizing Enzymes / Bailey, S.W. / Pike, D.C. / Ayling, J.E. -- Summary: Pterin and Folate Chemistry / Taylor, E. C. -- A Facile Method for Preparing 2,4-Diamino-5,8-Dideazapterins / Hynes, J.B. / Pathak, A. / Panos, C.H. / Okeke, C.C. -- Synthesis of 5,10-Dideaza-5,6,7,8-Tetrahydrofolic Acid (DDATHF) and Analogs / Taylor, Edward C. / Wong, George S. K. / Fletcher, Stephen R. / Harrington, Peter J. / Beardsley, G. Peter / Shih, Chuan J. -- Synthetic Routes to Methotrexate- and Folic Acid-Polyglutamates of High Chemical and Enantiomeric Purity / Przybylski, M. / Renkel, R. / Fonrobert, P. -- In Vivo and In Vitro Evaluations of 5-[4-(Substituted Aryl)-1- Piperazinyl]-6-Alkyl-2,4-Pyrimidinediamines as Antitumor Agents / Werbel, L.M. / Hung, J. / Besserer, J.A. / Boritzki, T.J. / Leopold, W.R. / Fry, D.W. -- The Electrochemical Oxidation of Tetrahydropterin Derivatives / Kwee, S. -- A Practical Synthesis of (6R)- and (6S)-5,6,7,8-Tetrahydrobiopterins / Matsuura, Sadao / Sugimoto, Takashi / Murata, Shizuaki -- Chemical Synthesis and Properties of Quinonoid (6R)-Dihydrobiopterin / Matsuura, Sadao / Murata, Shizuaki / Sugimoto, Takashi -- Pyrazine-Ring Conformations of Tetrahydropterins and Quinonoid-Dihydropterins / Gready, J. E. -- Hydrogenation of Silylated Pterins in Benzene Solution / Boyle, P.H. / Kelly, M.J. -- N(5)-Alkylation of Polyacetylated 5,6,7,8-Tetrahydroneopterins / Antoulas, S. / Viscontini, M. -- Oxidations Photosensitized by Pterins and Diaminopteridines / Aubailly, M. / Santus, R. -- Structure of the Riboflavin Synthase/Lumazine Synthase Complex. Arrangement and Chain Folding of Subunits in the Icosahedral Capsid / Ladenstein, R. / Bartunik, H. D. / Schneider, M. / Huber, R. / Schott, K. / Bacher, A. -- Use of C18 Silica Cartridges to Purify and Characterize Pterins / Jacobson, K. Bruce / Ferre, J. -- Synthesis and Structure-Activity Relationships of Tetrahydrobiopterin Cofactor Analogues / Bigham, E. C. / Smith, G. K. / Reinhard, J. F. -- Synthesis, Purification and Properties of a New Class of Pteridine Derivatives / Ferré, J. / Yim, J. J. / Pfleiderer, W. / Jacobson, K.B. -- Summary: Chemistry of Pterins / Hynes, John B. -- Section B: Biosynthesis and Biochemistry of Pterins -- Biosynthesis of Tetrahydrobiopterin and Related Compounds in Drosophila melanogaster / Brown, G.M. / Primus, J.P. / Switchenko, A.C. -- Tetrahydrobiopterin Biosynthesis in Man / Curtius, H.-Ch. / Takikawa, S. / Niederwieser, A. / Ghisla, S. -- The Biosynthesis of Tetrahydrobiopterin in the Bovine Adrenal Medulla / Smith, Gary K. / Duch, David S. / Nichol, Charles A. -- The Biosynthesis of Tetrahydrobiopterin in Rat Brain / Milstien, Sheldon / Kaufman, Seymour -- Summary: Biosynthesis of Tetrahydrobiopterin / Brown, G.M. -- The Metabolic Role of Tetrahydrobiopterin / Kaufman, Seymour -- Spectroscopic Studies on Tyrosine 3-Monooxygenase from Bovine Adrenal Medulla: A Blue Iron Protein / Haavik, J. / Andersson, K.K. / Petersson, L. / Flatmark, T. -- Queuine-Lacking tNRAs in Relation to the Grade of Malignancy of Specific Tumors, Possible Role of Pteridines / Kersten, W. / Zubrod, E. / Emmerich, B. / Maubach, P. -- Pteridine Synthesis During Interaction of Interleukin 2 with T Lymphocytes - Modulator Function in IL-2 Signal Transmission / Ziegler, I. / Ellwart, J. / Schwulera, U. -- Section C: Biology of Pterins -- Dopamine Neurons in Culture: A Model System for Examination of Neuronal Biopterin Metabolism / Kapatos, Gregory / Chiodo, Louis A. -- Role of the Pituitary in the Regulation of Tetrahydrobiopterin Biosynthesis in the Non-Neuronal Tissues / Duch, D.S. / Bowers, S.W. / Nichol, C.A. -- Distribution of GTP Cyclohydrolase-I, Neopterin, and Biopterin in the Human Brain / Nagatsu, T. / Sawada, M. / Akino, M. / Masada, M. / Sugimoto, T. / Matsuura, S. -- Enzymatic Synthesis of 6,7-Dimethyl-8-Ribityllumazine / Bacher, A. / Neuberger, G. / Volk, R. -- A Model for Hyperphenylal aninaemia Due to Tetrahydrobiopterin Deficiency / Cotton, R.G.H. -- The Effect of Biopterin and its Reduced Derivatives on Cultured Human Lymphocytes / Webber, S. / Jaye, D.L. -- Developmental Aspects of Biopterin Metabolism in Human / Dhondt, J.L. / Hayte, J.M. / Forzy, G. / Delcroix, M. / Farriaux, J.P. / Largillière, C. -- Screening for Biopterin Defects Among Hyperphenylalaninemic Patients: Report of a Canadian Program After 3 Years / Mohyuddin, F. / Rupar, C.A. / Evers, M.C. -- Need for Therapy in utero of Fetuses with Tetrahydrobiopterin Deficiency / Sawada, Y. / Shintaku, H. / Suyama, I. / Isshiki, G. / Hase, Y. / Okano, Y. / Yamamoto, H. / Tsuruhara, T. / Oura, T. -- Pteridine Metabolism in Patients Suffering from Amyotrophic Lateral Sclerosis / Zeitler, H.-J. / Andondonskaja-Renz, B. / Küther, G. / Struppler, A. -- Urinary Neopterin in Cocaine-Abusing Individuals / Guynn, R.W. / Biehl, K. / Merrill, D.K. / Krajewski, K.J. -- Features of Neopterin Determination in Clinical Use / Werner, E.R. / Fuchs, D. / Hausen, A. / Reibnegger, G. / Wachter, H. / Huber, J.F.K. -- Diurnal Variation in Urinary Pterin Excretion in Man / Pheasant, Anne E. -- The Relationship between Pteridines and Colour Dimorphism in the Scorpion Fly / Tsusué, M. / Nakagoshi, M. -- Pteridine Biosynthesis and Manifestation of Pigment Phenotypes in Normal and Neoplastic Cells of Goldfish in Vitro / Masada, M. / Matsumoto, J. / Akino, M. -- Light Depending Regulations of Pteridines in the Retina / Cremer-Bartels, G. / Krause, K. -- Differences in the Regulation of Tetrahydrobiopterin Biosynthesis in Neuronal Cells in Culture / Woolf, J. H. / Nichol, C. A. / Duch, D. S. -- NADPH- and NADH-Specific Dihydropteridine Reductase in Teratocarcinoma Cells in Culture: Effect of Nerve Growth Factor Treatment of the Cells on the Enzyme Activities / Nakanishi, Nobuo / Ozawa, Kenichi / Yamada, Shozo / Akiba, Kaoruko / Sato, Naoyoshi -- Reversibility of α-Diketo Reductase Activity by Sepiapterin Reductase / Katoh, S. / Sueoka, T. / Yamada, S. -- Non-Enzymatic Synthesis of "Drosopterins" from Dihydropterin and 2-Amino-4-0xo-6-Acetyl-3H, 9H-7,8-Dihydropyrimido [4,5b] [1,4]Diazepine / Paton, D. R. / Brown, G. M. -- BH4 Biosynthesis: 1H-NMR Evidence for the Pyruvoyl-Tetrahydropterin-Synthase Catalyzed Formation of 6-Pyruvoyl-Tetrahydropterin from Dihydroneopterin Triphosphate / Ghisla, S. / Takikawa, S. / Steinerstauch, P. / Hasler, Th. / Curtius, H.-Ch. -- Catabolism of Tetrahydrobiopterin in Man / Niederwieser, A. / Matasović, A. / Kuster, Th. / Staudenmann, W. / Pfleiderer, W. / Scheibenreiter, Susanne -- Pteridine Biosynthesis in Human Amniocytes and Chorionic Villi / Ferré, J. / Naylor, E.W. -- The Role of the Kidney in Biopterin Metabolism / Dhondt, J.L. / Hayte, J.M. / Noël, C. / Dracon, M. / Lelievre, G. / Tacquet, A. -- Tetrahydrobiopterin-Producing Enzyme Activities in Liver of Animals and Man / Hasler, Th. / Niederwieser, A. -- Effect of 3-Hydroxykynurenine on GTP Cyclohydrolase Activity and on the Pteridine Pattern in Drosophila melanogaster / Silva, Francisco J. / Ménsua, José L. -- Tetrahydrobiopterin Metabolism in Normal Brain, Senile Dementia of the Alzheimer Type and Down's Syndrome / Anderson, J.M. / Hamon, C.G.B. / Armstrong, R.A. / Blair, J.A. -- Summary: Biology of Pteridines / Parniak, M.A. -- Biosynthesis of Pteridines and Metabolism of Aromatic Amino Acids in Drosophila melanogaster / Bel, Y. / Ferré, J. -- The Inhibition of tRNA Transglycosylases by Pteridines as a Mean for Metabolic Control of Gene Expression / Kersten, H. / Aschhoff, H.-J. / Böhm, P. / Zubrod, E. -- Modulation of Dopamine Synthesis by Nerve Terminal Autoreceptors: A Role for Tetrahydrobiopterin? / Galloway, Matthew P. / Levine, Robert A. -- A Monoclonal Antibody Reacting with All Three Aromatic Amino Acid Hydroxylases / Cotton, R.G.H. / Jennings, I.G. / Kuskinsky, R. / Chow, C.W. / Haan, E. / McAdam, W.J. / Bell, C. / Morgan, F. / Nakata, H. / Cuello, A.C. -- Alterations in Cofactor-Dependent Activity of Phenylalanine Hydroxylase as a Function of pH / Parniak, M.A. / Kaufman, S. -- Interaction of a Monoclonal Antibody with Rat Liver Phenylalanine Hydroxylase / Parniak, M.A. / Jennings, I.G. / Cotton, R.G.H. -- The Effect of Dietary Iron on the Activity of Rat Liver Phenylalanine Hydroxylase / Davis, Michael D. / Kaufman, Seymour -- Strict Requirement of Fe2+ for Tryptophan 5-Monooxygenase from Mouse Mastocytoma, P-815 / Hasegawa, Hiroyuki / Ichiyama, Arata -- Tetrahydrobiopterin and the Regulation of Catecholamine Synthesis in Bovine Adrenal Medullary Chromaffin Cells / Levine, Robert A. / Kelner, Katrina L. -- Cofactor Activity of Tetrahydropterins for Tyrosine Hydroxylation in PC12 Pheochromocytoma Cells / Duch, D.S. / Reinhard, J.F. / Edelstein, M.P. / Chao, J.Y. / Nichol, C.A. -- Summary: Pterin-Dependent Biological Reactions / Levine, Robert A. -- Tetrahydrobiopterin Deficiency. Analysis from an International Survey / Dhondt, Jean-Louis -- Cofactor Specificity of 6,6-Disubstituted Tetrahydropterins, and their Potential in the Treatment of Neurological Disorders / Ayling, J.E. / Bailey, S.W. / Dillard, S.B. -- Normal Concentrations of Tetrahydrobiopterin in the Cerebrospinal Fluid of Patients with Dihydropteridine Reductase Deficiency / Hyland, K. / Smith, I. / Howells, D. W. -- Prenatal Diagnosis of Tetrahydrobiopterin Deficiency / Niederwieser, A. / Shintaku, H. / Hasler, Th. / Curtius, H.-Ch. / Lehmann, H. / Leupold, D. / Guardamagna, O. / Ponzone, A. / Schmidt, H. -- Defective Biopterin Biosynthesis in a Chinese Infant / Scriver, C.R. / Clow, C.L. / Kaplan, P. / Watters, G.V. / Laberge, C. / Mohyuddin, Fazl / Milstien, S. -- Correlation of Dihydropterine Reductase Cross Reacting Material with Non-Responsiveness to a Tetrahydrobiopterin Load / Cotton, R.G.H. / Jennings, I.G. / Bracco, G. / Ponzone, A. / Guardamagna, O. -- Neopterin and Dihydroneopterins in Serum of Controls and Patients with Various Diseases. Circadian Rhythm of Neopterin. Influence of Cortisol? / Rokos, H. / Frisius, Hildegard / Kunze, R. -- Neopterin Content and GTP-Cyclohydrolase Activity of Blood Cells of Controls and Patients Positive for LAV/HTLV-III Antibodies / Rokos, K. / Kunze, R. / Lange, W. / Koch, M.A. -- Neopterin in Bone Marrow Transplant Monitoring: An Improvement in its Use for GVHD Diagnosis / Mura, P. / Piriou, A. / Reiss, D. / Guilhot, F. / Benz-Lemoine, E. / Tanzer, J. -- Importance of Neopterin Determination in Individuals at Risk for AIDS / Fuchs, D. / Hausen, A. / Reibnegger, G. / Werner, E.R. / Wachter, H. / Blecha, H.G. / Roessler, H. / Unterweger, B. / Hinterhuber, H. / Hengster, P. / Schulz, T. / Dierich, M.P. / Renner, D. -- Biochemical Aspects of Pteridines During Interaction Between Activated T-Lymphocytes and Monocytes/Macrophages in Patients / Andondonskaja-Renz, B. / Zeitler, H.-J. -- Summary: Pterins and Human Illness / Fuchs, D. / Wachter, H. -- Section D: Folates and Pterins in Mammalian Tissues -- Folates in Tissues and Cells. Support for a "Two-Tier" Hypothesis of Regulation of One-Carbon Metabolism / Krumdieck, Carlos L. / Eto, Isao -- Elevation in the Rate of Cellular Folate Catabolism in Mid-Pregnancy in the Rat / Scott, J.M. / Wilson, E. / Weir, D.G. -- The Red Blood Cell as a Storage Site for Folic Acid / Steinberg, Stephen E. -- Methotrexate and Biopterin Levels in Blood Following Low Dose Administration for Psoriasis / Leeming, R.J. / Conlon, R.M. / Pollock, A. / Kumari, J. -- Summary: Folates in Tissue and Cells / Priest, D.G. -- Folate Rescue of N2O-Induced Inhibition in Murine Lymphocytes / Hilton, J. G. / Cooper, B. A. / Lapp, W. -- Folate Absorption and Metabolism in Alcohol Fed Rats / Noronha, J.M. / Kesavan, V. -- Chronic and Acute Effects of Ethanol on Renal Clearance and Urinary Excretion of Folate in Rats / McMartin, K.E. / Eisenga, B.H. / Collins, T.D. / Bairnsfather, L. -- Effect of Chronic Anticonvulsant Treatment on Folate Concentrations in the Rat / Carl, G.F. -- Alcohol Induced Methyl Group Wastage as a Possible Cause of Fatty Liver / Molloy, A.M. / Scott, J.M. / Weir, D.G. -- Folate Catabolism in the Syrian Golden Hamster / Al-Haddad, D. / Pheasant, A.E. / Blair, J.A. / Hamon, C.G.B. -- The Level of Folate Catabolism in Normal Human Populations, Suggesting that the Current Level of RDA for Folate is Excessive / McPartlin, J.M. / Weir, D.G. / Courtney, G. / McNulty, H. / Scott, J.M. -- Microdetermination of Folate Monoglutamates in Serum by High-Performance Liquid Chromatography with Electrochemical Detection / Iwai, K. / Inoue, K. / Kohashi, M. -- Folate Status in Psychiatric Patients: Relationship with CSF Neurotransmitter Metabolites / Bottiglieri, T. / Laundy, M. / Carney, M.W.P. / Chary, T.K.N. / Toone, B.K. / Reynolds, E.H. -- Folate, Thiamine and Vitamin B12 Levels in Outpatient Epileptics / Botez, M. I. -- Effect of Aspirin Ingestion on Folate Bioavailability Evaluated by Rat Liver Bioassay / Hoppner, K. / Lampi, B. -- Brush Border Pteroylpolyglutamate Hydrolase in Pig Jejunum / Chandler, C.J. / Wang, T.T.Y. / Halsted, C.H. -- Use of CI-920 in the Characterization of the Methotrexate Transport Defect in a Resistant Human Leukemic CCRF-CEM Cell Line / Jansen, G. / Schornagel, J.H. / Rijksen, G. / Gier, J. de -- The Effect of Oxidative Stress on Methotrexate (MTX) Transport by Rat Hepatocytes / Rhee, Myung S. / Pupons, Aiga / Nimec, Zenia / Galivan, John -- Folate Transport by Pig Intestinal Brush Border Vesicles / Reisenauer, A.M. -- Depressed Folate Transfer Across the Mammary Gland Secondary to Iron Deficiency in the Rat / O’Connor, D.L. / Picciano, M.F. / Sherman, A.R. / Burgert, S.L. -- Studies on the Mechanism of Folate Transport in Isolated Hepatocytes / Horne, Donald W. -- Analysis of the pH and Na+ Dependence of Intestinal Folate Transport / Rosenberg, I.H. / Zimmermann, J. / Selhub, J. -- Intestinal Transport of Folate. Characterization of the Transport Events at the Brush Border and the Basolateral Membranes / Said, H. M. / Strum, W. B. -- The Synthesis of Some Novel Probes of Folate Transport and Binding / Kempton, Robert J. / Sams, Laura A. / Harpring, Kathleen M. / Smith, Amy G. / Kohrs, Frank / Price, Elmer / Freisheim, James H. -- Photoaffinity Probes of Dihydrofolate Reductase and of the Membrane Carrier for Folate Analogues / Price, E.M. / Smith, P.L. / Freisheim, J.H. -- The Use of Biosynthetic Radioactive Folylpolyglutamates to Study Intestinal Digestion and Absorption of Folate / Darcy-Vrillon, B. / Selhub, J. / Rosenberg, I.H. -- Selective Inhibition of Bacterial Carboxypeptidase G and Pancreatic Conjugase by 2-Mercaptomethylglutaric Acid / Kalman, T.I. / Nayak, V.K. / Reddy, A.R.V. -- Summary: Folate Absorption and Transport / Rosenberg, I.H. -- Studies on Glycine-N-Methyltransferase / Wagner, Conrad / Cook, Robert J. -- Folate Binding Protein from Pediococcus Cerevisiae Strains Possessing Active Transport Systems for Folates / Mandelbaum-Shavit, Frederika -- Immobilized Folate Binding Protein from Cow's Milk Used for Quantitation of Folate / Hansen, S.I. / Nexø, E. / Holm, J. -- Characterization of Rabbit Antibodies Against the Folate Binding Protein from Cow's Milk / Høier-Madsen, M. / Hansen, S.I. / Holm, J. -- Section E: Enzymology of Folates -- 1. Thymidylate Synthase -- Genetic and Chemical Studies on Thymidylate Synthase / Maley, Frank / Chu, Frederick K. / West, Deborah K. / Maley, Gladys F. -- Active Site Probes of Thymidylate Synthetase / Broom, Arthur D. / Yang, In-Young -- Resonance-Raman Spectroscopic Identification of the Transient Intermediates Formed in the Native Ternary Complex with Thymidylate Synthase / Fitzhugh, A.L. / Kaufman, S. / Fodor, S. / Spiro, T.G. -- The Reversal of the Cytotoxicity of Folate-Based Thymidylate Synthase Inhibitors in Cultures L1210 Cells / Jackman, A.L. / Calvert, A.H. / Moran, R.G. -- Thymidylate Synthase Activity in the Tapeworm, Hymenolepis diminuta / Cieśla, J. / Zieliński, Z. / Rode, W. / Machnicka, B. -- Identification of Poly G Bound to Thymidylate Synthase / Thorndike, J. / Kisliuk, R.L. -- Methotrexate Derivates of Deoxyuridylate Showing Inhibitory Properties for Thymidylate Synthetase from Lactobacillus casei / Webber, S. / Nazarbaghi, R. / Whiteley, J.M. -- Comparison of Thymidylate Synthase Isolated from Mouse Normal and Tumour Tissues / Zieliński, Z. / Cieśla, J. / Kędzierska, B. / Rode, W. -- Thymidylate Synthase Interaction with Analogues of dUMP and 5-Fluorid- 2'-Deoxyuridine-5'-Phosphate with Modified Phosphate Groups / Rode, W. / Kędzierska, B. / Kulikowski, T. / Shugar, D. -- An In Vitro System for Studies on Inhibition of the Thymidylate Cycle / Rebandel, H. / Gaumont, Y. / Kisliuk, R.L. -- N10-Propargyl-5,8-Dideazafolic Acid Polyglutamates as Inhibitors of Thymidylate Synthase and their Intracellular Formation / Sikora, E. / Jackman, A.L. / Newell, D.R. / Harrap, K.R. / Calvert, A.H. / Jones, T.R. / Pawelczak, K. / Rzeszotarska, B. -- Sequential CMF in Metastatic Breast Cancer: An Attempt to Increase Effectiveness by Means of Biochemical Modulation / Pronzato, P. / Repetto, L. / Amoroso, D. / Ardizzoni, A. / Bertelli, G. / Conte, P.F. / Fusco, V. / Gulisano, M. / Rosso, R. -- Model Studies of Thymidylate Synthase Reaction / Meij, Paul F.C. van der / Hilhorst, Ellen / Chen, Tjoe B.R.A. / Waard, Eduard R. de / Pandit, Upendra K. -- Synergistic Interaction Between Folinic Acid and the Fluoropyrimidines / Keyomarsi, Khandan / Moran, Richard G. -- 2. Methionine Biosynthesis -- Methionine Biosynthesis / Matthews, Rowena G. / Jencks, David A. / Frasca, Verna / Matthews, Keith D. -- Impaired Formylation and Uptake of Tetrahydrofolate by Rat Small Gut Following Cobalamin Inactivation / Perry, Janet / Deacon, Rosemary / Lumb, M. / Chanarin, I. -- Heterogeneity in Functional Methionine Synthase Deficiency / Watkins, David / Rosenblatt, David S. -- Summary: Methionine Biosynthesis / Stokstad, E. L. R. -- 3. Folyl Polyglutamate Synthetase -- Folylpoly-γ-glutamate Synthetase / Shane, B. -- Cytotoxic Effects and Inhibition of Folylpolyglutamate Synthetase by Folate Analogs Containing 2, Omega-Diaminoalkanoic Acids / McGuire, John J. / Piper, James R. -- An Upstream Gene Regulates the Expression of Folylpolyglutamate Synthetase-Dihydrofolate Synthetase in Escherichia coli / Bognar, Andrew L. / Osborne, Charles / Shane, Barry -- Occurence and Synthesis of Pteroyl-γ-glutamyl-γ-glutamyl-poly-α-glutamates in Escherichia coli / Ferone, R. / Hanlon, M. / Singer, S. / Hunt, D. -- Summary: Folylpolyglutamate Synthesis / Cossins, Edwin A. -- The Metabolism of Pteroylpolyglutamates / Kisliuk, Roy L. -- Dihydropteroyl Hexaglutamate and T4 Phage Baseplate Assembly / Szewczyk, Boguslaw / Szewczyk, Krystyna / Kozloff, Lloyd M. -- Effects of Polyglutamylation on Folate Cofactor and Antifolate Activity in the Thymidylate Synthase Cycle of Permeabilized Murine Leukemia L1210 Cells / Kalman, Thomas I. -- Summary: Pteroylpolyglutamate metabolism / MacKenzie, R. E. -- 4. Dihydrofolate Reductase -- Recent Advances in the Study of Dihydrofolate Reductase / Blakley, Raymond L. / Appleman, James R. -- The 2.8 Å Structure of a Type II Plasmid Encoded Dihydrofolate Reductase / Matthews, D.A. / Smith, S.L. / Baccanari, D.P. / Burchall, J.J. / Oatley, S.J. / Kraut, J. -- Mutations in the Human Dihydrofolate Reductase / Schweitzer, B. I. / Srimatkandada, S. / Dube, S.K. / Bertino, J.R. -- Theoretical Studies of the Structure, Conformational and Electronic Properties of Anticancer Folate Inhibitors / Welsh, W.J. / Cody, V. -- Binding of Inhibitors with Spin-Labeled Side Chains to Dihydrofolate Reductase (DHFR) from Several Species / Blakley, R.L. / Kulinski, R.F. / Appleman, J.R. / Piper, J.R. -- Affinity Labeling of Dihydrofolate Reductase with an Iodoacetyl Lysine Analogue of Methotrexate / Delcamp, T.J. / Rosowsky, A. / Smith, P.L. / Wright, J.E. / Freisheim, J.H. -- Escherichia coli Dihydrofolate Reductase Isolated as a Folate Complex / Joyner, S.S. / Baccanari, D.P. -- Dihydrofolate Reductase from Soybean Seedlings / Ratnam, S. / Delcamp, T.J. / Freisheim, J.H. -- Use of Antibodies as Structural Probes of Dihydrofolate Reductases and Enzyme-Ligand Complexes / Ratnam, M. / Delcamp, T.J. / Freisheim, J.H. -- Cooperativity in Inhibitor Binding to Neisseria gonorrhoeae Dihydrofolate Reductase / Tansik, Robert L. / Baccanari, David P. -- pH Studies of the Reaction Catalyzed by Dihydrofolate Reductase from E. coli / Morrison, John F. / Stone, Stuart R. -- Effects of pH on Reactions Catalyzed by Dihydrofolate Reductase from Chicken Liver / Morrison, John F. / Stone, Stuart R. -- Unconjugated Dihydropterin Substrates for Bovine Liver Dihydrofolate Reductase / Smith, G.K. / Banks, S.D. / Bigham, E.C. / Nichol, C.A. -- Enzymatic Characterization of Recombinant Human Dihydrofolate Reductase Produced in E. coli / Stüber, D. / Bujard, H. / Hochuli, E. / Kocher, H.P. / Kompis, I. / Talmadge, K. / Weibel, E.K. / Winkler, F.K. / Then, R.L. -- Levels of Folates and Methotrexate Polyglutamate Formation in Chinese Hamster Ovary Cells Lacking Dihydrofolate Reductase / Joannon, P. / Goldberg, H. / Whitehead, V.M. / Rosenblatt, D.S. / Vuchich, M.J. / Beaulieu, D. -- Methotrexate in Adjuvant Arthritis / Galivan, John / Rehder, Mary-Catherine / Kerwar, Suresh -- Computer Graphic Modeling in Drug Design: Conformational Analysis and Active-Site Modeling of Lipophilic Diaminopyrimidines / Cody, Vivian -- Effect of Antifolates 10-Methyl- and 10-Ethyl-10-Deaza-Aminopterin on a Human Breast Cancer Cell Line / Mandelbaum-Shavit, Frederika -- Biochemical and Cytotoxic Effects of the Erythro- and Threo-Isomers of Gamma-Fluoro-Methotrexate / McGuire, John J. / Galivan, John / Coward, James K. -- 5. Other Folate Enzymes -- C1-Tetrahydrofolate-Synthase, a Multifunctional Enzyme Involved in Purine Metabolism, and its Related Monofunctional Enzymes / Shannon, K.W. / Whitehead, T.R. / Staben, C. / Rabinowitz, J.C. -- The Interaction of Tetrahydropteroylpolyglutamates with 10-Formyltetra-Hydrofolate Synthetase / Schirch, V. / Strong, B. / Joshi, G. / Lura, R. / Dennis, T. -- Summary: Folates in C 1 Metabolism / Schirch, V. -- Chemical Aspects of Dimethylglycine Dehydrogenase and Sarcosine Dehydrogenase / Cook, Robert J. / Gettins, Peter / Wagner, Conrad -- Folate Normalizes Elevated PRPP Levels of Folate-Deficient HL-60 Cells / Ghitis, J. / Schreiber, C. / Waxman, S. -- Stereochemistry of Hydride Transfer to NADP+ by Methylene Tetrahydrofolate Dehydrogenase from Pig Liver / Green, Jacalyn / Matthews, Rowena G. / MacKenzie, Robert E. -- The Effects of Thyroxine Status on Hepatic Levels of 10-Formyltetrahydrofolic Acid: NADP Oxidoreductase / Keating, J.M. / Choe, H. / Stokstad, E.L.R. -- Interaction of Homocysteine with Methionine Synthetase / Lewis, Garry P. -- Minor Form of Human Hepatic Betaine: Homocysteine S-Methyltransferase / Skiba, William E. / Wells, Marilyn S. / Mangum, John H. / Awad, William M. -- Studies of Cobalamin-Dependent Methionine Synthase from Escherichia coli B / Frasca, Verna / Dunham, William R. / Sands, Richard H. / Stephenson Riazzi, Barbara / Matthews, Rowena G. -- Inhibition of Methylenetetrahydrofolate Reductase by Adenosyl Methionine / Jencks, David A. / Matthews, Rowena G. -- Regulation of Folate Homeostasis / Osborne, C. / Lowe, K. / Shane, B. / Cichowicz, D. J. / Sussman, D. / Milman, G. -- An Endogenous Inhibitor of Neurospora Folylpolyglutamate Synthetase / Cossins, E.A. / Chan, P.Y. -- Folylpolyglutamate Hydrolase from Beef Liver / Vickers, P. J. / Di Cecco, R. / Pristupa, Z. B. / Scrimgeour, K. G. -- Use of Mammalian Cell Transformation to Isolate Genomic Sequences Encoding Human Folylpolyglutamate Synthetase (FPGS) and Dihydrofolate Reductase (DHFR) / Taylor, Shirley M. / Davidson, Norman / Moran, Richard G. -- Section F: Methotrexate and Other Anti-Folates -- Antifolates: Expanding Horizons in 1986 / Chabner, Bruce A. / Allegra, Carmen J. / Baram, Jacob -- Deaza Derivatives of Tetrahydrofolic Acid. A New Class of Folate Antimetabolite / Beardsley, G. Peter / Taylor, Edward C. / Grindey, Gerald B. / Moran, Richard G. -- Structure Activity Relationships of Novel Triazine Antifolates / Dias Selassie, C. / Hansch, C. / Zheng, Y.C. / Zhu, H. / Khwaja, T. / Freisheim, J. / Delcamp, T.J. -- The Effect of 7-Hydroxymethotrexate on the Antitumor Activity and Host Toxicity of Methotrexate In Vivo / Newton, P.A. / Finch, R.A. / Avery, T.L. -- Summary: The Biology of Antifolates / Gallivan, J. -- Crystal Structure of Methotrexate and its Conformational Comparison with Related Structures / Sutton, Paul A. / Cody, Vivian -- Effect of Methotrexate on Reduced Folates and Thymidylate Synthesis During Growth of L1210 Cells / Priest, D.G. / Kesavan, V. / Doig, M.T. -- Dihydrofolate Reductase from Human Osteosarcoma Cells Resistant to Methotrexate / Sato, Judith K. / DeFrank, Theodore J. -- Evidence for Direct Inhibition of Metabolic Pathways as a Mechanism of Action of Methotrexate / Allegra, C.J. / Baram, J. / Chabner, B.A. -- Inhibition of Dihydrofolate Reductase and Thymidylate Synthase by Methotrexate Polyglutamate Analogues Lacking "Internal" α-Carboxyl Groups / Rosowsky, A. / Forsch, R.A. / Wick, M.M. / Freisheim, J.H. / Danenberg, P.V. / Kalman, T.I. -- The Antifolate Activity of Poly-γ-Glutamyl Derivatives of Methotrexate, 10-Deazaaminopterin and 10-Ethyl-10 Deazaaminopterin / Kisliuk, R.L. / Gaumont, Y. / Kumar, P. / Nair, M.G. / Kaufman, B.T. -- Polyglutamation of the Thymidylate Synthase Inhibitor, N10-Propargyl-5,8-Dideazafolic Acid (CB 3717), in Organs of Ehrlich Ascites Carcinoma-Bearing Mice / Manteuffel-Cymborowska, M. / Kamińska, B. / Grzelakowska-Sztabert, B. -- 5,8-Dideazafolates as Substrates for Folypoly-γ-Glutamate Synthetase from Pig Liver / Hynes, J. B. / Cichowicz, D. J. / Shane, B. -- 5-Substituted-5-Deaza Analogues of Classical Antifolates / Piper, J. R. / McCaleb, G. S. / Montgomery, J. A. / Sirotnak, F. M. -- Azido-Substituted Antifolate Drugs: Synthesis, Structure, and Activity / Bryant, P.K. / Wong, K.P. / Colby, J. / Schwalbe, C.H. / Stevens, M.F.G. / Griffin, R.J. / Bliss, E.A. -- Summary: Novel Antifolates / Jackson, Robert C. -- Author Index -- Subject Index -- List of Participants -- Backmatter