Human Embryonic Stem Cell Protocols

Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC).- Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos.- Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts.- In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Protocol.- Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency.- Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells.- Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Standardized Culture Method.- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR.- Culture and Preparation of Human Embryonic Stem Cells for Proteomics-Based Applications.- A Two- and Three-Dimensional Approach for Visualizing Human Embryonic Stem Cell Differentiation.- Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions.- Study of Gap Junctions in Human Embryonic Stem Cells.- hESC Engineering by Integrase-Mediated Chromosomal Targeting.- Human Embryonic Stem Cell Differentiation on Periodontal Ligament Fibroblasts.- Generation of Neural Crest Cells and Peripheral Sensory Neurons from Human Embryonic Stem Cells.- Embryonic Stem Cells as a Model for Studying Melanocyte Development.- In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells.- Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds.- Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells.- Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo.- Genetic Manipulation of Human Embryonic Stem Cells.- Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media.- Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions.