Für diesen Artikel ist leider kein Bild verfügbar.

Protein Crystallography

A Concise Guide
Buch | Hardcover
152 Seiten
2008
Johns Hopkins University Press (Verlag)
978-0-8018-8806-9 (ISBN)
81,65 inkl. MwSt
The proteome remains a mysterious realm. Researchers have determined the structures of only a small fraction of the proteins encoded by the human genome. Crystallography continues to be the primary method used to determine the structures of the remaining unknown proteins. This imaging technique uses the diffraction of X-rays to determine a protein's three-dimensional molecular structure. Drawing on years of research and teaching experience, Eaton E. Lattman and Patrick J. Loll use clear examples and abundant illustrations to provide a concise and accessible primer on protein crystallography. Discussing the basics of diffraction, the behavior of two- and three-dimensional crystals, phase determination (including MIR and MAD phasing and molecular replacement), the Patterson function, and refinement, Lattman and Loll provide a complete overview of this important technique, illuminated by physical insights. The crisp writing style and simple illustrations will provide beginner crystallographers with a guide to the process of unraveling protein structure.

Eaton E. Lattman is chair of the Department of Biophysics at the Johns Hopkins University and editor-in-chief of the journal Proteins: Structure, Function, and Genetics. Patrick J. Loll is the director of the Biochemistry Graduate Program at Drexel University College of Medicine.

Preface
1. Introduction
1.1. What Is X-ray Crystallography?
1.2. A Quick Look at Protein Crystals
1.3. Noncrystalline Specimens
1.4. Summary
Further Reading
2. A Physical Understanding of Diffraction
2.1. What Is Diffraction?
2.2. Diffraction from One-Dimensional Crystals
2.3. Reconstructing Images from Diffraction Patterns
2.4. Summary
Further Reading
3. Diffraction from Three-Dimensional Crystals
3.1. The Electron Density Function in Three Dimensions
3.2. Calculating the Diffraction Pattern from a Known Structure
3.3. Summary
Further Reading
4. Phase Determination by Isomorphous Replacement
4.1. Measuring the Phases
4.2. MAD Phasing
4.3. Fitting Models to Experimental Electron Density Maps
4.4. Summary
Further Reading
5. The Patterson Function
5.1. Definition of the Patterson Function
5.2. Using the Patterson Function to Locate Atoms
5.3. Summary
Further Reading
6. Phasing with Partially Known Structures
6.1. Difference Fourier Maps
6.2. Molecular Replacement
6.3. Summary
Further Reading
7. Crystallographic Refinement
7.1. Refinement Improves the Model
7.2. Least-Squares Refinement
7.3. Summary
Further Reading
Glossary
Index

Erscheint lt. Verlag 23.6.2008
Zusatzinfo 46 Illustrations, black and white
Verlagsort Baltimore, MD
Sprache englisch
Maße 152 x 229 mm
Gewicht 340 g
Themenwelt Studium 1. Studienabschnitt (Vorklinik) Physiologie
Studium 2. Studienabschnitt (Klinik) Humangenetik
Naturwissenschaften Biologie
Naturwissenschaften Chemie Organische Chemie
Naturwissenschaften Geowissenschaften Mineralogie / Paläontologie
ISBN-10 0-8018-8806-9 / 0801888069
ISBN-13 978-0-8018-8806-9 / 9780801888069
Zustand Neuware
Haben Sie eine Frage zum Produkt?
Mehr entdecken
aus dem Bereich

von Hans-Christian Pape; Armin Kurtz; Stefan Silbernagl

Buch (2023)
Thieme (Verlag)
110,00
Lehrbuch

von Erwin-Josef Speckmann; Jürgen Hescheler …

Buch | Hardcover (2024)
Urban & Fischer in Elsevier (Verlag)
89,00