G ProteinsTechniques of Analysis

David R. Manning, Ph.D. Department of Pharmacology University of Pennsylvania School of Medicine Philadelphia, PA

Preface Expression and Purification of G Protein a Subunits in E. scherichia coli - M. Linder Purification of Recombinant G Protein a and bg Subunits from Sf9 Cells-T. Kozasa Expression and Functional Analysis of G Protein a Subunits in Mammalian Cells...-C. H. Berlot Identification of Regulators and Targets of G Protein a Subunits Using the Yeast Two-Hybrid System-J.L. Glick, T. E. Meigs, and P. J. Casey Functional Analysis of G Protein bg Subunits...-K. Yan, O. Kisselev, A. Pronin and N. Gautam Immunological Techniques of Analysis-C. A. Chen and D. R. Manning GTPase-Activating Proteins (GAPs) for Heterotrimeric G Proteins- J. Wang, Y. Tu, S. Mukhopadhyay, P. Chidiac, G. Biddlecome and E. M. Ross Fatty Acid Acylation of G Protein a Subunits- P.B.Wedegaetner ADP-Ribosylation of G Proteins with Cholera Toxin or Pertussis Toxin- M. J. Woolkalis Analysis of Receptor.G Protein coupling with PTX-Risistant Analogues of G(i in subscript) and G(o in subscript)- A. Mortiz and R. Taussig Microinjection of Antibodies Neutralizing G. Protein Function-V. J. LaMorte, J. R. Feramisco and J. L. Meinkoth Agonist-Promoted (35S)GTPgS-Binding as a Probe of Receptor-G Protein Communication in Reconstituted Sf9 Cells- A.J. Barr and D. R. Manning Methods of Sequestering bg in the Intact Cell- G. J. Della Rocca, R. J. Lefkowitz and W. J. Koch In Vitro and In Vivo Analysis of Heterotrimeric G-Protein Signaling via DNA/RNA Antisense Strategies-H. Wang, X. Liu, Z. Cheng, H. Ling Li and C. C. Malbon