DNA Damage Detection In Situ, Ex Vivo, and In Vivo
Humana Press Inc. (Verlag)
978-1-60327-408-1 (ISBN)
Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.
In Situ Detection of Apoptosis by the TUNEL Assay: An Overview of Techniques.-Combination of TUNEL Assay with Immunohistochemistry for Simultaneous Detection of DNA Fragmentation and Oxidative Cell Damage.- EM-ISEL: A Useful Tool to Visualize DNA Damage at the Ultrastructural Level.- In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase.- In Situ Ligation: a Decade and a Half of Experience.-In Situ Ligation Simplified: Using PCR Fragments for Detection of Double-Strand DNA Breaks in Tissue Sections.- 5’OH DNA Breaks in Apoptosis and their Labeling by Topoisomerase-Based Approach.- Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image-Cytometry.- Fluorochrome-Labeled Inhibitors of Caspases (FLICA): Convenient In Vitro and In Vivo Markers of Apoptotic Cells for Cytometric Analysis.- Combining Fluorescent In Situ Hybridization with the Comet Assay for Targeted Examination of DNA Damage and Repair. Simultaneous Labeling of Single- and Double-Strand DNA Breaks by DNA Breakage Detection-FISH (DBD-FISH).- Co-Localization of DNA Repair Proteins with UV-induced DNA Damage in Locally Irradiated Cells.- Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects at High and Low-Frequencies. - Quantifying Etheno-DNA Adducts in Human Tissues, White Blood Cells and Urine by Ultrasensitive 32P-postlabeling and Immunohistochemistry.- ELISpot Assay as a Tool to Study Oxidative Stress in Peripheral Blood Mononuclear Cells.- Cytokinesis-Block Micronucleus Cytome assay in Lymphocytes.- Buccal Micronucleus Cytome Assay.- -H2AX Detection in Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts and Skin.- Immunologic Detection of Benzo(a)pyrene-DNA Adducts.- Non-Invasive Assessment of Oxidatively Damaged DNA: Liquid Chromatography-Tandem Mass Spectrometry Analysis of Urinary 8-Oxo-7,8-dihydro-2’-deoxyguanosine.- Assessing sperm DNA fragmentation with the Sperm Chromatin Dispersion (SCD) test.
Reihe/Serie | Methods in Molecular Biology ; 682 |
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Zusatzinfo | XIII, 313 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete |
Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik | |
ISBN-10 | 1-60327-408-1 / 1603274081 |
ISBN-13 | 978-1-60327-408-1 / 9781603274081 |
Zustand | Neuware |
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