Gene Therapy Protocols

Preparation and Quantification of Pseudotyped Retroviral Vector.- Production of Retroviral Vectors for Clinical Use.- Methods for the Production of Helper-Dependent Adenoviral Vectors.- Methods for the Production of First Generation Adenoviral Vectors.- Large-Scale Production of Recombinant Adeno-Associated Viral Vectors.- Construction and Production of Recombinant Herpes Simplex Virus Vectors.- Plasmid-Based Gene Transfer in Mouse Skeletal Muscle by Electroporation.- Chitosan Nanoparticle-Mediated Gene Transfer.- PEG–PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues.- Non-Viral Gene Delivery with Cationic Liposome–DNA Complexes.- Applications of Lentiviral Vectors in Noninvasive Molecular Imaging.- Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia.- Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells.- Assessment of CFTR Function after Gene Transfer In Vitro and In Vivo.- Oncolytic Adenoviruses for Cancer Gene Therapy.- Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Therapy.- Prevention of Type 1 Diabetes in NOD Mice by Genetic Engineering of Hematopoietic Stem Cells.- Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells.- Nanoparticle-Mediated Gene Delivery to the Lung.- Retroviral-Mediated Gene Therapy for the Differentiation of Primary Cells into a Mineralizing Osteoblastic Phenotype.- In Vivo siRNA Delivery to the Mouse Hypothalamus Shows a Role of the Co-Chaperone XAP2 in Regulating TRH Transcription.- Efficient Retroviral Gene Transfer to Epidermal Stem Cells.