WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction

Acknowledgements; Abbreviations; 1. Introduction; 2. Collection and examination of human semen; 2A. Standard procedures; 2B. Optional tests; 2C. Research tests; 3. Sperm preparation techniques; 4. Quality control in the andrology laboratory; 5. Sperm-cervical mucus interactions; Appendices. IA. Reference values of semen variables; IB. Nomenclature for some semen variables; II. Safety guidelines for the andrology laboratory; III. Methods for detecting leucocytes; IV. Sperm vitality techniques; V. Papanicolau staining procedures modified for spermatozoa; VI. Shorr staining procedure for sperm morphology; VII. Rapid staining procedure for sperm morphology; VIII. Immunobead test; IX. Mixed antiglobulin reaction test (MAR test); X. Measurement of zinc in seminal plasma; XI. Measurement of fructose in seminal plasma; XII. Measurement of neutral a-glucosidase in seminal plasma; XIII. Sample instructions and record form for semen analysis; XIV. Computer-aided sperm analysis (CASA); XV. Protocols for the zona-free hamster test; XVI. Measurement of reactive oxygen species generated by sperm suspensions; XVII. Induced acrosome reaction assay; XVIII. Sperm preparation techniques; XIX. Quality control (QC) procedures; XX. Sample record form for cervical mucus scoring and postcoital test; XXI. Sperm-cervical mucus interaction: the capillary tube test; XXII. Basic requirements for an andrology laboratory; Bibliography; Index.