Transfusion and Hematopoietic Stem Cell

Introduction: the role of blood centers for haematopoetic stem cell transplantation. Part 1 Bone marrow and peripheral blood stem cell transplantation: current status of allogeneic bone marrow transplantation in Japan; collection and transplant of peripheral blood stem cells in children; peripheral blood stem cell transplantation for the treatment of haematologic malignancy; peripheral blood stem cell transplantation for the treatment of solid tumours; kinetics of peripheral blood stem cell collection and transplantation. Part 2 Efficient collection of peripheral blood stem cells: therapy-orientated strategies in collecting peripheral blood stem cells (PBSC); peripheral blood stem cell collection with multi-component apheresis system MCS-3p. Part 3 Characterization of haematopoietic stem cells in bone marrow, peripheral blood and umbilical cord blood: different responses of human marrow, peripheral blood and cord blood haemotopoietic progenitors of cytokines; purification of CD34-positive cells from umbilical cord blood; characterization of marrow, blood and umbilical cord-derived stem cells after cryopreservation; expression of adhesion molecules on human haematopoietic progenitor cells; purification and characterization of haematopoietic stem cells in cord blood. Part 4 Signal transduction in stem cells: signal transduction in stem cells. Part 5 Placenta blood stem cell transplantation: placenta/umbilical cord blood stem cells for narrow reconstitution in man. Part 6 Regulation of stem cell differentiation: differentiation and proliferation of murine lymphohemopoietic progenitors. Part 7 In vivo expansion of haematopoietic stem cells: peripheral blood stem cell transplantation - the UCLH experience; autologous, syngeneic, and allogeneic transplantation of peripheral blood stem cells; expression of adhesion molecules on haematopoietic progenitor cells mobilized by G-CSF; in vivo expansion of peripheral blood stem cells by injection of rhG-CSF in healthy volunteer donors; High-dose chemotherapy with thiotepa mitoxantrone and cyclophosphamide (TMC), with autologous progenitor cell support in the treatment of breast cancer. Part 8 In vitro expansion of haematopoietic stem cells: signal transduction in stem cell proliferation haematopoietic reconstitution of murine haematopoietic progenitor cells expanded in vitro; in vitro expansion of human peripheral blood CD43+ cells and transitional change of proliferative and differentiative capacity. Part 9 Umbilical cord blood stem cells: isolation, expansion and transplantation; methods of cell sedation using monoclonal antibodies selection and transplantation of CD34+ cells using the isolex immunomagnetic cell separation systems; autologous bone marrow transplantation using positive selection of human haematopoietic progenitor cells in the treatment of breast cancer; mobilization, separation and expansion of peripheral blood progenitor cells; in vitro expansion of cord blood. (Part contents)